Experiment set8IT062 for Phaeobacter inhibens DSM 17395

Cytidine nitrogen source

Group: nitrogen source
Media: DinoMM_noNitrogen_HighNutrient_SucroseC + Cytidine (5 mM), pH=7.2
Culturing: Phaeo_ML1, tube, Aerobic, at 25 (C), shaken=200 rpm
Growth: about 5.4 generations
By: Mark on 1/20/2015
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM Sucrose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 7 genes in this experiment

For nitrogen source Cytidine in Phaeobacter inhibens DSM 17395

For nitrogen source Cytidine across organisms

SEED Subsystems

Subsystem	#Specific
High affinity phosphate transporter and control of PHO regulon	1
Mercuric reductase	1
Mercury resistance operon	1
Oxidative stress	1
Phosphate metabolism	1
Rubrerythrin	1
Thioredoxin-disulfide reductase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phenylmercury acetate degradation	2	1	1