Experiment set8IT059 for Klebsiella michiganensis M5al

Compare to:

Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM

Group: carbon source
Media: RCH2_defined_noCarbon + D-Glucose (55.5 mM) + 0.5x Fungal_growth_media_mCAFEs_v1_noCarbon
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Fungal_growth_media_mCAFEs_v1_noCarbon 0.5x includes: 0.1 g/L Magnesium Sulfate Heptahydrate, 0.45 g/L Potassium phosphate dibasic, 0.1 g/L Potassium Chloride, 0.5 g/L Ammonium Nitrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate (final concentrations)

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Glucose in Klebsiella michiganensis M5al

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Thiamin biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I 2 2 1
superpathway of thiamine diphosphate biosynthesis I 10 10 2
superpathway of thiamine diphosphate biosynthesis II 11 9 2
thiazole component of thiamine diphosphate biosynthesis I 6 6 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) 7 3 1