Experiment set8IT053 for Phaeobacter inhibens DSM 17395
L-Leucine nitrogen source
Group: nitrogen sourceMedia: DinoMM_noNitrogen_HighNutrient_SucroseC + L-Leucine (5 mM), pH=7.2
Culturing: Phaeo_ML1, tube, Aerobic, at 25 (C), shaken=200 rpm
Growth: about 5.8 generations
By: Mark on 1/20/2015
Media components: 20 g/L Sea salts, 0.1 g/L Potassium phosphate monobasic, 20 mM Sucrose, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For nitrogen source L-Leucine in Phaeobacter inhibens DSM 17395
For nitrogen source L-Leucine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Aromatic amino acid interconversions with aryl acids | 1 |
| Fructose utilization | 1 |
| Ketoisovalerate oxidoreductase | 1 |
| Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Valine, leucine and isoleucine degradation
- Pyruvate metabolism
- Propanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| acetate and ATP formation from acetyl-CoA III | 1 | 1 | 1 |
| acetate conversion to acetyl-CoA | 1 | 1 | 1 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| ethanol degradation II | 3 | 3 | 1 |
| L-isoleucine biosynthesis V | 3 | 2 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| chitin deacetylation | 4 | 2 | 1 |
| L-leucine degradation I | 6 | 5 | 1 |
| superpathway of bitter acids biosynthesis | 18 | 3 | 3 |
| adlupulone and adhumulone biosynthesis | 6 | 1 | 1 |
| colupulone and cohumulone biosynthesis | 6 | 1 | 1 |
| lupulone and humulone biosynthesis | 6 | 1 | 1 |
| reductive glycine pathway of autotrophic CO2 fixation | 9 | 6 | 1 |
| cis-geranyl-CoA degradation | 9 | 2 | 1 |