Experiment set8IT046 for Pseudomonas syringae pv. syringae B728a

library passed through aphids in food, 72 hours

Group: aphid
Media:
Culturing: SyringaeB728a_ML2
By: Tyler Helmann on 3/18/19

Specific Phenotypes

For 40 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Flagellum	16
Flagellar motility	10
Bacterial Chemotaxis	5
Two-component regulatory systems in Campylobacter	2
Campylobacter Iron Metabolism	1
Cobalamin synthesis	1
Coenzyme B12 biosynthesis	1
DNA repair, bacterial DinG and relatives	1
Hemin transport system	1
Methionine Biosynthesis	1
Ton and Tol transport systems	1
Transcription initiation, bacterial sigma factors	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Methionine metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• One carbon pool by folate
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-methionine salvage from L-homocysteine	3	2	1
L-methionine biosynthesis III	4	4	1
L-methionine biosynthesis I	5	3	1
Salmonella enterica serotype O:3,10 O antigen biosynthesis	5	2	1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1)	6	2	1
Salmonella enterica serotype O:9 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:2 O antigen biosynthesis	6	2	1
Salmonella enterica serotype O:9,46 O antigen biosynthesis	6	2	1
Porphyromonas gingivalis O-LPS antigen biosynthesis	6	2	1
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	13	2
superpathway of L-homoserine and L-methionine biosynthesis	8	6	1
folate transformations III (E. coli)	9	9	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	7	1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis	9	2	1
folate transformations II (plants)	11	10	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	13	1
folate transformations I	13	9	1
superpathway of L-methionine salvage and degradation	16	7	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
adenosylcobalamin biosynthesis I (anaerobic)	36	29	2
aspartate superpathway	25	22	1
adenosylcobalamin biosynthesis II (aerobic)	33	31	1