Experiment set8IT043 for Pseudomonas fluorescens FW300-N2E2

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Trisodium citrate dihydrate carbon source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3842 -6.1 -4.2 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_64 -6.0 -4.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3253 -5.8 -4.0 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4826 -5.5 -7.5 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3751 -5.4 -12.6 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4597 -5.4 -9.0 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_4596 -5.4 -9.0 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_77 -5.4 -7.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4825 -5.3 -8.1 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3630 -5.3 -7.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_71 -5.2 -7.1 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4047 -5.2 -5.0 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_2277 -5.1 -3.5 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_5176 -5.0 -9.5 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5175 -4.7 -14.8 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3782 -4.7 -4.6 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3839 -4.7 -4.6 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3752 -4.7 -21.7 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_3783 -4.7 -6.4 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_5014 -4.6 -2.1 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_2286 -4.4 -4.4 FIG00953287: hypothetical protein compare
Pf6N2E2_3661 -4.3 -4.2 hypothetical protein compare
Pf6N2E2_3252 -4.3 -10.3 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3841 -4.2 -5.7 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4564 -4.2 -8.7 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_66 -4.0 -10.3 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_5177 -4.0 -11.7 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_2073 -3.9 -8.3 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3940 -3.8 -11.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4048 -3.8 -8.0 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_5156 -3.8 -2.6 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_3948 -3.7 -11.5 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_302 -3.7 -8.0 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3932 -3.6 -6.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3610 -3.3 -5.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4638 -3.3 -3.9 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_4138 -3.2 -16.5 Uncharacterized transporter, similarity to citrate transporter conserved
Pf6N2E2_4092 -3.1 -4.4 FIG00954395: hypothetical protein compare
Pf6N2E2_4071 -3.1 -4.4 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4077 -2.9 -3.4 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_3160 -2.9 -10.3 Multicopper oxidase conserved
Pf6N2E2_4360 -2.9 -11.9 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_3248 -2.7 -4.8 Probable transmembrane protein compare
Pf6N2E2_50 -2.7 -8.7 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5618 -2.7 -11.3 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites conserved
Pf6N2E2_4612 -2.7 -6.5 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_5579 -2.7 -7.0 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_3908 -2.6 -6.7 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_3659 -2.6 -5.2 Protein fixF compare
Pf6N2E2_4447 -2.5 -14.0 Cytochrome c family protein compare
Pf6N2E2_2853 -2.5 -10.3 Iron-regulated protein A precursor conserved
Pf6N2E2_5666 -2.5 -11.6 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_3251 -2.4 -9.2 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_5616 -2.4 -5.3 outer membrane component of uptake system, probably for ferrous iron (from data) conserved
Pf6N2E2_5331 -2.4 -6.7 ATPase, AFG1 family compare
Pf6N2E2_3984 -2.4 -10.5 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_63 -2.3 -4.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_2833 -2.3 -7.0 Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3) compare
Pf6N2E2_5527 -2.3 -2.6 Cold shock protein CspC compare
Pf6N2E2_2513 -2.3 -5.6 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5006 -2.2 -8.3 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_4009 -2.2 -11.3 GGDEF domain protein compare
Pf6N2E2_2465 -2.2 -5.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_3835 -2.2 -4.5 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_2834 -2.2 -6.0 Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3) compare
Pf6N2E2_5617 -2.2 -8.6 Iron-regulated protein A precursor compare
Pf6N2E2_3170 -2.2 -5.4 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_3847 -2.1 -4.8 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_4369 -2.1 -2.4 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_5667 -2.1 -7.5 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_5111 -2.1 -2.3 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_4797 -2.0 -3.0 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_2218 -2.0 -5.4 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_5950 -2.0 -3.7 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_4372 -1.9 -5.0 FIG00460773: hypothetical protein compare
Pf6N2E2_5728 -1.9 -3.4 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_2473 -1.9 -1.8 Major outer membrane lipoprotein I compare
Pf6N2E2_5305 -1.8 -13.1 Fe(3+) dicitrate transport protein FecA (from data) conserved
Pf6N2E2_3607 -1.8 -7.9 3'-to-5' exoribonuclease RNase R compare
Pf6N2E2_5307 -1.8 -5.2 FIG006045: Sigma factor, ECF subfamily conserved
Pf6N2E2_4759 -1.8 -11.9 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_2510 -1.8 -4.8 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_2830 -1.7 -3.6 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) compare
Pf6N2E2_5257 -1.7 -8.0 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_4987 -1.7 -1.7 MaoC-like domain protein compare
Pf6N2E2_6140 -1.7 -1.1 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_2839 -1.7 -10.1 Pca regulon regulatory protein PcaR compare
Pf6N2E2_2219 -1.7 -2.4 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_2831 -1.7 -2.9 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2) compare
Pf6N2E2_5258 -1.7 -7.4 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3899 -1.6 -7.1 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_2835 -1.6 -2.4 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) compare
Pf6N2E2_3762 -1.6 -6.4 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_5155 -1.6 -3.3 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_2753 -1.6 -3.0 HtrA protease/chaperone protein compare
Pf6N2E2_2914 -1.5 -8.0 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) (from data) compare
Pf6N2E2_2913 -1.5 -6.8 Transcriptional regulator PobR, AraC family compare
Pf6N2E2_3206 -1.5 -1.6 hypothetical protein compare
Pf6N2E2_2837 -1.5 -7.2 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12) compare
Pf6N2E2_2074 -1.5 -1.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_2088 -1.5 -1.6 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf6N2E2_4422 -1.5 -2.0 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_58 -1.5 -1.8 tRNA-dihydrouridine synthase C (EC 1.-.-.-) compare
Pf6N2E2_5679 -1.5 -2.5 hypothetical protein compare
Pf6N2E2_1705 -1.4 -1.9 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_5520 -1.4 -5.2 Sensory box histidine kinase compare
Pf6N2E2_2836 -1.4 -4.0 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12) compare
Pf6N2E2_3349 -1.4 -2.8 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_5031 -1.4 -4.8 hypothetical protein compare
Pf6N2E2_3255 -1.4 -3.9 YrbA protein compare
Pf6N2E2_2838 -1.3 -6.8 4-hydroxybenzoate transporter compare
Pf6N2E2_5785 -1.3 -1.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_865 -1.3 -1.9 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_2217 -1.3 -4.0 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_5766 -1.3 -1.9 Quaternary ammonium compound-resistance protein SugE compare
Pf6N2E2_3282 -1.3 -2.1 FIG005080: Possible exported protein compare
Pf6N2E2_1441 -1.3 -2.4 Uncharacterized oxidoreductase ydgJ (EC 1.-.-.-) compare
Pf6N2E2_5219 -1.3 -6.3 FIG140336: TPR domain protein compare
Pf6N2E2_1490 -1.3 -2.3 hypothetical protein compare
Pf6N2E2_5243 -1.3 -1.9 hypothetical protein compare
Pf6N2E2_4414 -1.3 -1.8 FIG00953808: hypothetical protein compare
Pf6N2E2_70 -1.3 -3.8 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_2274 -1.2 -4.8 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_2251 -1.2 -4.0 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) compare
Pf6N2E2_3933 -1.2 -6.1 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_4351 -1.2 -7.5 Predicted signal transduction protein compare
Pf6N2E2_5613 -1.2 -2.6 FIG00953674: hypothetical protein compare
Pf6N2E2_1281 -1.2 -3.2 ATPase involved in DNA repair compare
Pf6N2E2_4827 -1.2 -4.2 Lipoate-protein ligase A compare
Pf6N2E2_2285 -1.2 -1.5 FIG00954079: hypothetical protein compare
Pf6N2E2_5749 -1.2 -3.3 hypothetical protein compare
Pf6N2E2_5794 -1.2 -2.5 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_4417 -1.2 -4.0 Protein of unknown function DUF484 compare
Pf6N2E2_4615 -1.2 -2.1 type IV pili signal transduction protein PilI compare
Pf6N2E2_3300 -1.1 -2.5 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_3667 -1.1 -1.0 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_2022 -1.1 -5.4 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_4136 -1.1 -5.0 Aromatic ring-cleaving dioxygenase compare
Pf6N2E2_1294 -1.1 -1.9 Carbon starvation protein A compare
Pf6N2E2_178 -1.1 -1.4 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_3665 -1.1 -1.1 serine/threonine protein kinase compare
Pf6N2E2_4416 -1.1 -2.3 Tyrosine recombinase XerC compare
Pf6N2E2_4611 -1.1 -3.1 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_4591 -1.0 -3.0 tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) compare
Pf6N2E2_4206 -1.0 -1.2 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_5103 -1.0 -0.9 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_2282 -1.0 -3.5 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf6N2E2_1676 -1.0 -1.4 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_5130 -1.0 -1.6 hypothetical protein compare
Pf6N2E2_3763 -1.0 -1.9 hypothetical protein compare
Pf6N2E2_2447 -1.0 -4.1 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_1776 -1.0 -2.2 Chemotaxis protein CheD compare
Pf6N2E2_3969 -1.0 -1.4 Membrane-fusion protein compare
Pf6N2E2_3014 -1.0 -1.9 P2-like prophage tail protein X compare
Pf6N2E2_5703 -1.0 -3.7 outer membrane protein H1 compare
Pf6N2E2_5452 -1.0 -2.8 hypothetical protein compare
Pf6N2E2_3947 -1.0 -1.4 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_4338 -1.0 -4.1 Type VI secretion lipoprotein/VasD compare
Pf6N2E2_3786 -1.0 -2.8 Twin-arginine translocation protein TatC compare
Pf6N2E2_3872 -0.9 -1.4 hypothetical protein compare
Pf6N2E2_5720 -0.9 -6.8 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_942 -0.9 -2.0 Mll6465 protein compare
Pf6N2E2_3351 -0.9 -2.6 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_3039 -0.9 -5.0 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_3335 -0.9 -6.7 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB compare
Pf6N2E2_2962 -0.9 -2.8 ABC transporter for L-Lysine, ATPase component (from data) compare
Pf6N2E2_4508 -0.9 -2.9 Transcriptional regulator, TetR family compare
Pf6N2E2_2065 -0.9 -2.6 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_2420 -0.9 -2.5 hypothetical protein compare
Pf6N2E2_613 -0.9 -1.3 lipoprotein, putative compare
Pf6N2E2_4614 -0.9 -2.3 twitching motility protein PilH compare
Pf6N2E2_1317 -0.9 -2.8 P-hydroxyphenylacetate hydroxylase C2:oxygenase component compare
Pf6N2E2_3876 -0.9 -2.9 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_4454 -0.9 -3.7 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_4994 -0.9 -2.4 Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18) compare
Pf6N2E2_2080 -0.9 -1.0 hypothetical protein compare
Pf6N2E2_3725 -0.9 -1.5 FIG085779: Lipoprotein compare
Pf6N2E2_5306 -0.9 -5.2 Iron siderophore sensor protein compare
Pf6N2E2_5001 -0.9 -1.3 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_5848 -0.9 -2.4 small component of pyruvate transporter (yjcH-like) (from data) compare
Pf6N2E2_1182 -0.9 -2.9 Transcriptional regulator, LysR family compare
Pf6N2E2_1981 -0.9 -4.0 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_4162 -0.9 -2.2 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_146 -0.9 -1.3 Transcriptional regulator, MarR family compare
Pf6N2E2_2549 -0.8 -1.6 hypothetical protein compare
Pf6N2E2_3658 -0.8 -4.4 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_5986 -0.8 -1.6 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3477 -0.8 -1.7 2-Oxobutyrate oxidase, putative compare
Pf6N2E2_2832 -0.8 -5.3 dicarboxylic acid transporter PcaT compare
Pf6N2E2_904 -0.8 -2.9 hypothetical protein compare
Pf6N2E2_5226 -0.8 -1.3 FIG00955589: hypothetical protein compare
Pf6N2E2_5013 -0.8 -2.4 Putative membrane protein compare
Pf6N2E2_4091 -0.8 -4.1 Oligopeptidase A (EC 3.4.24.70) compare
Pf6N2E2_1056 -0.8 -0.6 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_2519 -0.8 -0.9 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_5252 -0.8 -1.5 FIG00956396: hypothetical protein compare
Pf6N2E2_5665 -0.8 -3.3 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_712 -0.8 -2.9 hypothetical protein compare
Pf6N2E2_4120 -0.8 -5.5 Trk system potassium uptake protein TrkA compare
Pf6N2E2_370 -0.8 -3.0 Predicted enzyme of the cupin superfamily compare


Specific Phenotypes

For 18 genes in this experiment

For carbon source Trisodium citrate dihydrate in Pseudomonas fluorescens FW300-N2E2

For carbon source Trisodium citrate dihydrate across organisms