Experiment set8IT033 for Pseudomonas fluorescens FW300-N2E2

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Trisodium citrate dihydrate carbon source

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_2770 +3.8 31.0 Tricarboxylate transport membrane protein TctA conserved
Pf6N2E2_2769 +3.7 21.6 Tricarboxylate transport protein TctB conserved
Pf6N2E2_2252 +3.7 8.5 NreA-like protein compare
Pf6N2E2_5420 +2.9 17.8 Cytosol aminopeptidase PepA (EC 3.4.11.1) compare
Pf6N2E2_3942 +2.8 18.7 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_3192 +2.8 6.7 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_3271 +2.6 6.4 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_4418 +2.4 3.3 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_3941 +2.2 9.2 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_760 +2.1 15.8 Na+/H+ antiporter NhaB compare
Pf6N2E2_5160 +2.1 3.6 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_2253 +2.0 3.6 hypothetical protein compare
Pf6N2E2_5157 +2.0 13.5 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4365 +1.9 9.7 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_3261 +1.9 8.6 hypothetical protein compare
Pf6N2E2_4187 +1.8 5.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_5571 +1.7 3.5 hypothetical protein compare
Pf6N2E2_2764 +1.7 12.9 Tricarboxylate transport sensor protein TctE conserved
Pf6N2E2_5158 +1.7 7.0 FIG00954153: hypothetical protein compare
Pf6N2E2_5705 +1.7 2.4 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_3521 +1.7 9.6 Sensor histidine kinase/response regulator compare
Pf6N2E2_3191 +1.7 4.1 Serine acetyltransferase (EC 2.3.1.30) compare
Pf6N2E2_1596 +1.7 3.0 Periplasmic nitrate reductase component NapD compare
Pf6N2E2_2768 +1.6 12.4 Tricarboxylate transport protein TctC conserved
Pf6N2E2_2254 +1.5 4.1 Cell filamentation protein fic compare
Pf6N2E2_2765 +1.5 6.2 Tricarboxylate transport transcriptional regulator TctD conserved
Pf6N2E2_4803 +1.5 1.7 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_5153 +1.5 8.6 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_4601 +1.4 5.3 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_5554 +1.4 6.2 FIG023103: Predicted transmembrane protein compare
Pf6N2E2_5084 +1.3 10.0 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_4159 +1.3 3.8 Cytochrome c4 compare
Pf6N2E2_2340 +1.3 3.0 Transcriptional regulator, GntR family compare
Pf6N2E2_3033 +1.3 1.6 hypothetical protein compare
Pf6N2E2_2399 +1.3 7.1 Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase compare
Pf6N2E2_4277 +1.2 9.0 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_5555 +1.2 2.5 Cold shock protein CspA compare
Pf6N2E2_291 +1.2 6.6 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_4579 +1.2 1.7 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_3938 +1.2 9.6 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_4181 +1.2 1.7 Organic hydroperoxide resistance protein compare
Pf6N2E2_3612 +1.2 3.4 HflK protein compare
Pf6N2E2_3314 +1.2 8.5 Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2) compare
Pf6N2E2_546 +1.2 2.7 FIG00966904: hypothetical protein compare
Pf6N2E2_2524 +1.2 5.1 S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase compare
Pf6N2E2_3656 +1.2 7.3 Probable transcription regulator Mig-14 compare
Pf6N2E2_1797 +1.2 2.4 Transcriptional regulator compare
Pf6N2E2_1424 +1.1 2.0 transcriptional regulator, TetR family compare
Pf6N2E2_3312 +1.1 3.8 Dipeptide transport system permease protein DppC (TC 3.A.1.5.2) compare
Pf6N2E2_3114 +1.1 8.0 Transcriptional regulator, TetR family compare
Pf6N2E2_3073 +1.1 7.1 N-succinyl-L,L-diaminopimelate aminotransferase alternative (EC 2.6.1.17) conserved
Pf6N2E2_5884 +1.1 7.5 Sensor histidine kinase compare
Pf6N2E2_4279 +1.1 7.0 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_3792 +1.1 6.0 Glucans biosynthesis protein G precursor compare
Pf6N2E2_2675 +1.1 1.2 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_4278 +1.1 7.1 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_4072 +1.0 4.0 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_4494 +1.0 5.4 Adenylate cyclase (EC 4.6.1.1) compare
Pf6N2E2_5768 +1.0 3.2 DNA recombination-dependent growth factor C compare
Pf6N2E2_434 +1.0 1.9 hypothetical protein compare
Pf6N2E2_3328 +1.0 2.7 transcriptional regulator compare
Pf6N2E2_1215 +1.0 3.1 probable membrane protein YPO2362 compare
Pf6N2E2_5338 +1.0 7.3 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_579 +1.0 1.6 FIG00962753: hypothetical protein compare
Pf6N2E2_3311 +1.0 5.4 Dipeptide transport system permease protein DppB (TC 3.A.1.5.2) compare
Pf6N2E2_4121 +1.0 3.7 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_2114 +1.0 3.4 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_5337 +1.0 4.1 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_746 +1.0 1.5 FIG00958097: hypothetical protein compare
Pf6N2E2_3326 +1.0 2.1 hypothetical protein compare
Pf6N2E2_5676 +1.0 3.5 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_3655 +0.9 7.1 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_156 +0.9 1.4 Carbon storage regulator compare
Pf6N2E2_4451 +0.9 4.3 FIG139438: lipoprotein B compare
Pf6N2E2_3283 +0.9 5.2 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_5861 +0.9 4.9 FIG00953885: hypothetical protein compare
Pf6N2E2_3875 +0.9 1.7 FIG00953324: hypothetical protein compare
Pf6N2E2_3014 +0.9 2.0 P2-like prophage tail protein X compare
Pf6N2E2_3885 +0.9 2.3 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_1905 +0.9 2.9 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf6N2E2_3569 +0.9 1.7 hypothetical protein compare
Pf6N2E2_1412 +0.9 2.1 hypothetical protein compare
Pf6N2E2_445 +0.9 4.5 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_1032 +0.9 1.1 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_3494 +0.9 2.4 Acyl dehydratase compare
Pf6N2E2_4580 +0.9 4.5 FIG015287: Zinc protease compare
Pf6N2E2_1625 +0.9 2.6 Ferric reductase (1.6.99.14) compare
Pf6N2E2_1236 +0.8 1.6 Transcriptional regulator, LysR family compare
Pf6N2E2_3828 +0.8 3.3 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_2247 +0.8 2.1 Transcriptional regulator, AraC family compare
Pf6N2E2_2808 +0.8 3.4 LemA family protein compare
Pf6N2E2_2440 +0.8 1.3 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf6N2E2_2755 +0.8 1.6 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_3313 +0.8 2.5 Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2) compare
Pf6N2E2_5524 +0.8 6.5 Outer membrane porin, OprD family compare
Pf6N2E2_3638 +0.8 3.0 hypothetical protein compare
Pf6N2E2_5150 +0.8 4.0 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_5862 +0.8 0.9 Lipoprotein, putative compare
Pf6N2E2_4640 +0.8 1.3 hypothetical protein compare
Pf6N2E2_3293 +0.8 1.6 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) compare
Pf6N2E2_2791 +0.8 2.0 FIG00954883: hypothetical protein compare
Pf6N2E2_1043 +0.8 1.9 Transcriptional regulator, IclR family compare
Pf6N2E2_2278 +0.8 6.5 Transcription-repair coupling factor compare
Pf6N2E2_127 +0.8 1.6 AttE component of AttEFGH ABC transport system compare
Pf6N2E2_5242 +0.8 1.4 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_1660 +0.8 1.4 RND efflux system, membrane fusion protein CmeA compare
Pf6N2E2_3226 +0.8 1.6 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_3072 +0.8 5.0 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_3791 +0.8 5.3 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_2825 +0.8 2.9 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_4476 +0.8 3.2 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf6N2E2_2180 +0.7 2.6 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_3461 +0.7 1.0 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_4659 +0.7 3.8 Lipoprotein, putative compare
Pf6N2E2_923 +0.7 1.5 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_464 +0.7 1.9 Sensory histidine kinase QseC compare
Pf6N2E2_4551 +0.7 2.3 hypothetical protein compare
Pf6N2E2_3472 +0.7 3.2 Probable short-chain dehydrogenase compare
Pf6N2E2_1435 +0.7 2.3 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase compare
Pf6N2E2_692 +0.7 2.6 Glucarate dehydratase (EC 4.2.1.40) compare
Pf6N2E2_4773 +0.7 1.1 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_1605 +0.7 4.1 Carboxynorspermidine dehydrogenase, putative (EC 1.1.1.-) compare
Pf6N2E2_4843 +0.7 2.5 FIG00956941: hypothetical protein compare
Pf6N2E2_586 +0.7 4.0 Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-) compare
Pf6N2E2_1782 +0.7 1.7 STAS domain protein compare
Pf6N2E2_5906 +0.7 1.8 Flp pilus assembly protein, pilin Flp compare
Pf6N2E2_2970 +0.7 2.4 methylated-DNA--protein-cysteine methyltransferase-related protein compare
Pf6N2E2_4062 +0.7 2.2 hypothetical protein compare
Pf6N2E2_3980 +0.7 5.4 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_4752 +0.7 5.4 Malate synthase G (EC 2.3.3.9) compare
Pf6N2E2_2428 +0.7 2.1 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_5261 +0.7 1.0 FIG00956983: hypothetical protein compare
Pf6N2E2_5448 +0.7 4.1 hypothetical protein compare
Pf6N2E2_4464 +0.7 2.3 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_669 +0.7 2.3 Nuclease inhibitor compare
Pf6N2E2_1636 +0.7 4.0 Ferrichrome-iron receptor compare
Pf6N2E2_5556 +0.7 3.5 Aminomethyltransferase (EC 2.1.2.10) (from data) compare
Pf6N2E2_5017 +0.7 3.8 Phosphate starvation-inducible protein PhoH, predicted ATPase compare
Pf6N2E2_1784 +0.7 1.5 Methyl-accepting chemotaxis protein compare
Pf6N2E2_848 +0.7 2.5 hypothetical protein compare
Pf6N2E2_5573 +0.7 3.4 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_2627 +0.7 3.1 Flagellar basal-body rod protein FlgG compare
Pf6N2E2_1885 +0.7 2.3 Transcriptional regulator, AraC family compare
Pf6N2E2_3624 +0.7 3.8 Flagellar motor rotation protein MotB compare
Pf6N2E2_664 +0.7 1.2 Acetoin catabolism protein X compare
Pf6N2E2_1336 +0.7 2.2 AraC family transcription regulator compare
Pf6N2E2_3978 +0.7 5.1 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_2646 +0.7 1.1 FIG00953416: hypothetical protein compare
Pf6N2E2_870 +0.7 2.3 DNA-binding response regulator compare
Pf6N2E2_2699 +0.7 3.7 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_2010 +0.7 1.0 hypothetical protein compare
Pf6N2E2_1502 +0.7 1.0 Two-component system regulatory protein compare
Pf6N2E2_1238 +0.6 2.1 hypothetical protein compare
Pf6N2E2_2788 +0.6 1.3 Transcriptional regulator, AsnC family compare
Pf6N2E2_367 +0.6 2.1 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_567 +0.6 3.1 Universal stress protein family 7 compare
Pf6N2E2_3981 +0.6 5.5 T1SS secreted agglutinin RTX compare
Pf6N2E2_5794 +0.6 0.9 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_458 +0.6 4.1 Protein rarD compare
Pf6N2E2_649 +0.6 1.7 General secretion pathway protein L compare
Pf6N2E2_4477 +0.6 2.4 Z-ring-associated protein ZapA compare
Pf6N2E2_1463 +0.6 1.6 Isochorismate pyruvate-lyase (EC 4.-.-.-) compare
Pf6N2E2_2586 +0.6 1.1 Hpt domain protein compare
Pf6N2E2_4813 +0.6 4.8 Probable two-component sensor, near polyamine transporter compare
Pf6N2E2_2588 +0.6 1.5 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_4581 +0.6 2.9 FIG015547: peptidase, M16 family compare
Pf6N2E2_2509 +0.6 1.5 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_4480 +0.6 4.8 Xaa-Pro aminopeptidase (EC 3.4.11.9) compare
Pf6N2E2_423 +0.6 1.5 Putative phosphatase YfbT compare
Pf6N2E2_2602 +0.6 3.5 Flagellin protein FlaA compare
Pf6N2E2_3969 +0.6 1.1 Membrane-fusion protein compare
Pf6N2E2_6087 +0.6 1.5 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_5340 +0.6 2.6 Stringent starvation protein A compare
Pf6N2E2_2181 +0.6 0.9 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_5425 +0.6 0.6 hypothetical protein compare
Pf6N2E2_888 +0.6 2.2 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_172 +0.6 2.2 FIG00953292: hypothetical protein compare
Pf6N2E2_4969 +0.6 0.9 hypothetical protein compare
Pf6N2E2_2071 +0.6 2.0 hypothetical protein compare
Pf6N2E2_2519 +0.6 0.8 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_5869 +0.6 1.5 Helicase subunit of the DNA excision repair complex compare
Pf6N2E2_3134 +0.6 0.6 hypothetical protein compare
Pf6N2E2_2284 +0.6 1.7 Cell division inhibitor-related protein compare
Pf6N2E2_4599 +0.6 1.8 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_2622 +0.6 4.3 Flagellar hook-associated protein FlgL compare
Pf6N2E2_1124 +0.6 1.3 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_1551 +0.6 1.2 Transcriptional regulator compare
Pf6N2E2_827 +0.6 1.7 probable dioxygenase compare
Pf6N2E2_4272 +0.6 2.2 putative membrane protein compare
Pf6N2E2_2978 +0.6 3.1 Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase compare
Pf6N2E2_1699 +0.6 1.4 FIG00955832: hypothetical protein compare
Pf6N2E2_4103 +0.6 2.9 Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion compare
Pf6N2E2_3088 +0.6 0.7 hypothetical protein compare
Pf6N2E2_3979 +0.6 4.1 Type I secretion system ATPase, LssB family LapB compare
Pf6N2E2_2824 +0.6 3.9 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_640 +0.6 1.4 Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system compare
Pf6N2E2_1975 +0.6 1.8 Integrase regulator R compare
Pf6N2E2_4829 +0.6 2.8 OsmC/Ohr family protein compare
Pf6N2E2_4305 +0.6 0.7 FIG00959590: hypothetical protein compare
Pf6N2E2_3037 +0.6 4.0 RNA polymerase sigma factor RpoS compare


Specific Phenotypes

For 25 genes in this experiment

For carbon source Trisodium citrate dihydrate in Pseudomonas fluorescens FW300-N2E2

For carbon source Trisodium citrate dihydrate across organisms