Experiment set8IT033 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.8 x) + D-Glucose (22.2 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate

Specific Phenotypes

For 10 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem #Specific
Lipid A-Ara4N pathway ( Polymyxin resistance ) 2
DNA repair, UvrABC system 1
Multidrug Resistance Efflux Pumps 1
Peptidoglycan Biosynthesis 1
Type IV pilus 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
polymyxin resistance 6 5 5
di-trans,poly-cis-undecaprenyl phosphate biosynthesis 2 2 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 5 3
polyisoprenoid biosynthesis (E. coli) 5 5 1
juvenile hormone III biosynthesis I 6 1 1
peptidoglycan biosynthesis II (staphylococci) 17 12 2
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 2
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 2
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 12 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1