Experiment set8IT032 for Pseudomonas fluorescens FW300-N2E2
L-Leucine carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + L-Leucine (20 mM), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
Growth: about 4.1 generations
By: Mark on 1/21/2016
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 30 genes in this experiment
For carbon source L-Leucine in Pseudomonas fluorescens FW300-N2E2
For carbon source L-Leucine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine degradation
- Arginine and proline metabolism
- Propanoate metabolism
- Methane metabolism
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Ascorbate and aldarate metabolism
- Urea cycle and metabolism of amino groups
- Glutamate metabolism
- Tryptophan metabolism
- Taurine and hypotaurine metabolism
- Aminosugars metabolism
- Lipopolysaccharide biosynthesis
- Ether lipid metabolism
- Sphingolipid metabolism
- Pyruvate metabolism
- 1,2-Dichloroethane degradation
- Butanoate metabolism
- One carbon pool by folate
- Thiamine metabolism
- Riboflavin metabolism
- Nicotinate and nicotinamide metabolism
- Folate biosynthesis
- Nitrogen metabolism
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: