Experiment set8IT032 for Klebsiella michiganensis M5al

Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (1 x)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate

Specific Phenotypes

For 5 genes in this experiment

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	2
Biotin biosynthesis	1
Coenzyme B12 biosynthesis	1
Multidrug Resistance Efflux Pumps	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Porphyrin and chlorophyll metabolism
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Biotin metabolism
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
alkylnitronates degradation	2	2	1
nitrate reduction V (assimilatory)	2	1	1
biotin biosynthesis from 8-amino-7-oxononanoate I	4	4	1
biotin biosynthesis from 8-amino-7-oxononanoate II	4	3	1
biotin biosynthesis from 8-amino-7-oxononanoate III	5	3	1
biotin biosynthesis II	6	4	1
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	10	2
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	14	2
biotin biosynthesis I	15	15	1
adenosylcobalamin biosynthesis II (aerobic)	33	29	2
adenosylcobalamin biosynthesis I (anaerobic)	36	31	2