Experiment set8IT032 for Klebsiella michiganensis M5al
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
Group: supernatantMedia: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (1 x)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Specific Phenotypes
For 5 genes in this experiment
SEED Subsystems
Subsystem | #Specific |
---|---|
Nitrate and nitrite ammonification | 2 |
Biotin biosynthesis | 1 |
Coenzyme B12 biosynthesis | 1 |
Multidrug Resistance Efflux Pumps | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Porphyrin and chlorophyll metabolism
- Ubiquinone and menaquinone biosynthesis
- Histidine metabolism
- Tyrosine metabolism
- Tryptophan metabolism
- Benzoxazinone biosynthesis
- Naphthalene and anthracene degradation
- Biotin metabolism
- Carotenoid biosynthesis - General
- Nitrogen metabolism
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
alkylnitronates degradation | 2 | 2 | 1 |
nitrate reduction V (assimilatory) | 2 | 1 | 1 |
biotin biosynthesis from 8-amino-7-oxononanoate I | 4 | 4 | 1 |
biotin biosynthesis from 8-amino-7-oxononanoate II | 4 | 3 | 1 |
biotin biosynthesis from 8-amino-7-oxononanoate III | 5 | 3 | 1 |
biotin biosynthesis II | 6 | 4 | 1 |
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 10 | 2 |
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 14 | 2 |
biotin biosynthesis I | 15 | 15 | 1 |
adenosylcobalamin biosynthesis II (aerobic) | 33 | 29 | 2 |
adenosylcobalamin biosynthesis I (anaerobic) | 36 | 31 | 2 |