Experiment set8IT029 for Pseudomonas fluorescens FW300-N2C3

50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride

Group: resistance
Media: RCH2_defined_putrescine_20mM + P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride (50 percent), pH=7
Culturing: pseudo5_N2-C3_1_ML2B, 15 mL tube, aerobic, at 30 (C), shaken=225 rpm
By: Lauren_Andrew on 12/2/2016

Specific Phenotypes

For 11 genes in this experiment

For resistance P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2C3

For resistance P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Glutathione-regulated potassium-efflux system and associated functions	1
Polyamine Metabolism	1
Potassium homeostasis	1
Pyruvate Alanine Serine Interconversions	1
Queuosine-Archaeosine Biosynthesis	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Alanine and aspartate metabolism
• Lysine degradation
• beta-Alanine metabolism
• Propanoate metabolism
• Butanoate metabolism
• Fatty acid biosynthesis
• Methionine metabolism
• Valine, leucine and isoleucine degradation
• Lysine biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Aminophosphonate metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Caprolactam degradation
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine biosynthesis I	1	1	1
putrescine degradation II	4	4	3
β-alanine degradation II	2	2	1
4-aminobutanoate degradation II	2	2	1
ammonia assimilation cycle II	2	2	1
4-aminobutanoate degradation III	2	2	1
ammonia assimilation cycle I	2	2	1
putrescine degradation V	2	2	1
4-aminobutanoate degradation I	2	1	1
superpathway of ornithine degradation	8	8	3
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	10	4
superpathway of ammonia assimilation (plants)	3	3	1
ammonia assimilation cycle III	3	3	1
superpathway of 4-aminobutanoate degradation	3	2	1
L-aspartate degradation III (anaerobic)	3	2	1
L-aspartate degradation II (aerobic)	3	2	1
superpathway of L-arginine and L-ornithine degradation	13	12	4
GABA shunt II	4	2	1
GABA shunt I	4	1	1
β-alanine biosynthesis II	6	5	1
L-glutamate and L-glutamine biosynthesis	7	6	1
4-aminobutanoate degradation V	7	2	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of coenzyme A biosynthesis II (plants)	10	9	1
nicotine degradation I (pyridine pathway)	17	7	1