Experiment set8IT029 for Bifidobacterium breve UCC2003

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MRS + kanamycin Time24hr

Group: stress timecourse
Media: MRS + Kanamycin sulfate (0.03 mg/mL), pH=7
Culturing: Bifido_ML2, 1L bottle, Anaerobic, at 37 (C), shaken=0 rpm
By: Charlotte Brannon/Becca Culver on 10/21/2020
Media components: 56 mM D-Glucose, 10 g/L Proteose Peptone, 10 g/L Beef extract, 5 g/L Yeast Extract, 1 g/L Tween 80, 0.2 g/L Triammonium citrate, 0.5 g/L Sodium acetate, 0.2 g/L Potassium phosphate dibasic, 0.1 g/L Magnesium sulfate, 0.05 g/L Manganese (II) sulfate monohydrate, 8.25 mM L-Cysteine

Specific Phenotypes

For 2 genes in this experiment

For stress timecourse Kanamycin sulfate in Bifidobacterium breve UCC2003

For stress timecourse Kanamycin sulfate across organisms

SEED Subsystems

Subsystem #Specific
Methionine Biosynthesis 2
Cysteine Biosynthesis 1
Glycine and Serine Utilization 1
Methionine Degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-cysteine biosynthesis III (from L-homocysteine) 2 2 2
homocysteine and cysteine interconversion 4 3 3
superpathway of L-cysteine biosynthesis (fungi) 6 4 3
hydrogen sulfide biosynthesis II (mammalian) 6 4 3
superpathway of L-cysteine biosynthesis (mammalian) 5 3 2
taurine biosynthesis III 3 1 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 4 3
L-methionine biosynthesis I 5 3 1
L-methionine biosynthesis II 6 5 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 5 1
superpathway of L-homoserine and L-methionine biosynthesis 8 6 1
superpathway of L-methionine salvage and degradation 16 5 2
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 23 1