Experiment set8IT028 for Pseudomonas fluorescens FW300-N2E2

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Urea nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3248 -7.1 -4.9 Probable transmembrane protein compare
Pf6N2E2_4826 -6.6 -4.6 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3630 -6.3 -4.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4597 -6.3 -6.1 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3783 -6.2 -4.3 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4825 -6.1 -5.9 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3573 -6.0 -5.9 HupE-UreJ family metal transporter compare
Pf6N2E2_64 -6.0 -4.1 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_71 -6.0 -5.8 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3751 -5.9 -11.2 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4279 -5.7 -10.9 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_5175 -5.7 -10.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3826 -5.7 -3.9 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3842 -5.6 -5.4 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_5177 -5.6 -10.7 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_2258 -5.5 -9.2 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_5176 -5.5 -7.5 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5006 -5.4 -7.4 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_3253 -5.4 -6.4 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4596 -5.3 -10.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_77 -5.2 -5.0 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3610 -5.2 -7.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4047 -5.2 -5.0 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4564 -5.1 -12.8 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_3782 -5.0 -4.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3841 -5.0 -3.4 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3940 -5.0 -11.9 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3752 -4.9 -22.9 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_4278 -4.9 -13.3 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_2073 -4.8 -5.7 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3252 -4.8 -9.7 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3839 -4.7 -4.6 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_66 -4.7 -11.4 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_3571 -4.6 -5.8 Urease accessory protein UreF compare
Pf6N2E2_4048 -4.6 -9.3 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3552 -4.6 -10.1 Urease accessory protein UreD conserved
Pf6N2E2_3553 -4.6 -7.6 Urease (EC 3.5.1.5) (from data) conserved
Pf6N2E2_2897 -4.5 -12.0 Glucokinase (EC 2.7.1.2) compare
Pf6N2E2_4353 -4.5 -5.3 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_3072 -4.4 -12.9 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_3932 -4.4 -6.0 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3825 -4.4 -5.8 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_2885 -4.3 -5.9 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_3556 -4.2 -14.1 Urease (EC 3.5.1.5) (from data) compare
Pf6N2E2_1186 -4.0 -8.0 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3060 -4.0 -3.8 Outer membrane protein H precursor compare
Pf6N2E2_1187 -3.9 -10.0 Probable nikkomycin biosynthesis protein, carboxylase compare
Pf6N2E2_3554 -3.9 -11.4 GCN5-related N-acetyltransferase compare
Pf6N2E2_4372 -3.8 -6.4 FIG00460773: hypothetical protein compare
Pf6N2E2_5150 -3.8 -8.0 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_3572 -3.7 -14.4 Urease accessory protein UreG conserved
Pf6N2E2_4759 -3.6 -17.4 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_658 -3.6 -12.3 sensor histidine kinase compare
Pf6N2E2_3257 -3.6 -2.4 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_2218 -3.5 -5.7 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_3260 -3.4 -6.4 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_2261 -3.2 -6.4 VacJ-like lipoprotein precursor compare
Pf6N2E2_1189 -3.2 -8.2 Thioredoxin reductase (EC 1.8.1.9) compare
Pf6N2E2_2074 -3.2 -1.9 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_3258 -3.1 -4.7 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_1188 -3.1 -6.4 Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17) compare
Pf6N2E2_5156 -3.0 -3.5 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5780 -3.0 -11.4 Periplasmic protease compare
Pf6N2E2_4277 -3.0 -10.5 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_2274 -2.9 -5.9 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_5339 -2.9 -8.6 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_4611 -2.9 -5.5 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_3570 -2.9 -5.4 Urease accessory protein UreE conserved
Pf6N2E2_3251 -2.8 -12.7 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_1190 -2.8 -7.1 Glutamine-dependent 2-keto-4-methylthiobutyrate transaminase compare
Pf6N2E2_63 -2.8 -4.7 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_3938 -2.8 -14.1 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_3074 -2.8 -2.3 FIG00960973: hypothetical protein compare
Pf6N2E2_5014 -2.7 -2.0 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_3170 -2.7 -6.6 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_4638 -2.7 -4.4 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_4057 -2.5 -1.5 lipoprotein, putative compare
Pf6N2E2_4276 -2.5 -6.9 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_4416 -2.4 -3.7 Tyrosine recombinase XerC compare
Pf6N2E2_2588 -2.4 -4.5 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_50 -2.4 -6.5 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_3786 -2.3 -4.5 Twin-arginine translocation protein TatC compare
Pf6N2E2_3349 -2.2 -4.5 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_5338 -2.2 -9.0 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_5666 -2.2 -8.7 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_2574 -2.1 -2.8 Flagellar synthesis regulator FleN compare
Pf6N2E2_3885 -2.1 -2.9 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_5579 -2.1 -4.7 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_4352 -2.1 -3.3 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_3664 -2.1 -7.2 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_5219 -2.1 -6.1 FIG140336: TPR domain protein compare
Pf6N2E2_5337 -2.0 -3.9 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_3665 -2.0 -1.4 serine/threonine protein kinase compare
Pf6N2E2_5027 -2.0 -12.3 AMP nucleosidase (EC 3.2.2.4) compare
Pf6N2E2_2552 -2.0 -5.9 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_4009 -2.0 -8.7 GGDEF domain protein compare
Pf6N2E2_5527 -2.0 -2.6 Cold shock protein CspC compare
Pf6N2E2_3835 -2.0 -3.9 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_4160 -2.0 -2.0 Cytochrome c5 compare
Pf6N2E2_2217 -2.0 -4.8 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_4206 -1.9 -2.2 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_2527 -1.9 -4.8 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_3658 -1.9 -6.9 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_6140 -1.9 -1.1 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_3373 -1.9 -3.4 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_302 -1.8 -4.7 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_5190 -1.8 -10.6 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_2889 -1.8 -9.0 Glucose ABC transporter, ATP-binding subunit (EC 3.6.3.-) compare
Pf6N2E2_3264 -1.8 -2.9 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3666 -1.8 -3.9 InaA protein compare
Pf6N2E2_2586 -1.8 -2.5 Hpt domain protein compare
Pf6N2E2_4659 -1.8 -3.3 Lipoprotein, putative compare
Pf6N2E2_3984 -1.8 -10.0 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_2890 -1.7 -8.3 Glucose ABC transport system, inner membrane component 2 compare
Pf6N2E2_3521 -1.7 -7.6 Sensor histidine kinase/response regulator compare
Pf6N2E2_160 -1.7 -3.5 hypothetical protein compare
Pf6N2E2_3198 -1.7 -2.1 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_2888 -1.6 -9.2 Glucose-selective porin OprB compare
Pf6N2E2_5160 -1.6 -2.6 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_4818 -1.6 -5.4 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf6N2E2_5316 -1.6 -1.9 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_3460 -1.6 -1.7 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_2022 -1.6 -6.0 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_2340 -1.6 -5.0 Transcriptional regulator, GntR family compare
Pf6N2E2_2891 -1.6 -8.4 Glucose ABC transport system, inner membrane component 1 compare
Pf6N2E2_5494 -1.5 -4.2 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_3659 -1.5 -4.5 Protein fixF compare
Pf6N2E2_2863 -1.5 -6.1 Gluconate permease compare
Pf6N2E2_1191 -1.5 -5.9 Thiaminase II (EC 3.5.99.2) compare
Pf6N2E2_5536 -1.5 -1.8 Holliday junction DNA helicase RuvA compare
Pf6N2E2_2896 -1.5 -3.2 DNA-binding response regulator GltR, controls specific porins for the entry of glucose compare
Pf6N2E2_3393 -1.5 -6.9 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_2899 -1.5 -4.5 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_70 -1.4 -2.9 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_2506 -1.4 -9.0 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2465 -1.4 -4.6 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2507 -1.4 -5.8 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_37 -1.4 -3.6 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_4159 -1.3 -4.1 Cytochrome c4 compare
Pf6N2E2_5554 -1.3 -3.8 FIG023103: Predicted transmembrane protein compare
Pf6N2E2_4817 -1.3 -9.8 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_5261 -1.3 -1.2 FIG00956983: hypothetical protein compare
Pf6N2E2_292 -1.3 -6.8 ATP-dependent Clp protease ATP-binding subunit ClpA compare
Pf6N2E2_2753 -1.3 -2.6 HtrA protease/chaperone protein compare
Pf6N2E2_4146 -1.3 -2.0 Membrane lipoprotein lipid attachment site containing protein USSDB6D compare
Pf6N2E2_3661 -1.3 -4.6 hypothetical protein compare
Pf6N2E2_4579 -1.2 -1.6 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_3607 -1.2 -5.3 3'-to-5' exoribonuclease RNase R compare
Pf6N2E2_331 -1.2 -2.4 Putative translation initiation inhibitor, yjgF family compare
Pf6N2E2_3869 -1.2 -6.5 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_2893 -1.2 -4.0 hypothetical protein compare
Pf6N2E2_2892 -1.2 -7.1 Glucose ABC transport system, periplasmic sugar-binding protein compare
Pf6N2E2_2895 -1.2 -5.5 Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster compare
Pf6N2E2_2018 -1.2 -2.6 hypothetical protein compare
Pf6N2E2_2188 -1.2 -4.0 Predicted transcriptional regulator LiuR of leucine degradation pathway, MerR family compare
Pf6N2E2_5676 -1.2 -2.3 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_5188 -1.2 -4.5 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_2474 -1.2 -6.4 ErfK/YbiS/YcfS/YnhG family protein compare
Pf6N2E2_6099 -1.2 -1.5 tRNA-Glu-TTC compare
Pf6N2E2_2010 -1.1 -1.4 hypothetical protein compare
Pf6N2E2_863 -1.1 -4.7 Transcriptional regulator, IclR family compare
Pf6N2E2_5806 -1.1 -2.5 hypothetical protein compare
Pf6N2E2_1776 -1.1 -2.5 Chemotaxis protein CheD compare
Pf6N2E2_1932 -1.1 -3.9 Transcriptional regulator, IclR family compare
Pf6N2E2_2455 -1.1 -5.0 FIG00953957: hypothetical protein compare
Pf6N2E2_5155 -1.1 -2.5 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_682 -1.1 -4.7 Predicted regulator PutR for proline utilization, GntR family compare
Pf6N2E2_1156 -1.1 -3.1 hypothetical protein compare
Pf6N2E2_1606 -1.1 -4.7 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_2117 -1.0 -6.5 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
Pf6N2E2_4824 -1.0 -2.7 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_1893 -1.0 -1.9 hypothetical protein compare
Pf6N2E2_5739 -1.0 -1.2 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf6N2E2_2223 -1.0 -2.5 hypothetical protein compare
Pf6N2E2_5103 -1.0 -0.7 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_3077 -1.0 -2.1 FIG138056: a glutathione-dependent thiol reductase compare
Pf6N2E2_5564 -1.0 -7.3 Membrane protein glpM conserved
Pf6N2E2_2795 -1.0 -1.6 putative exported protein compare
Pf6N2E2_170 -1.0 -1.6 lipoprotein, putative compare
Pf6N2E2_1445 -1.0 -1.9 Glutathione S-transferase compare
Pf6N2E2_5508 -1.0 -2.0 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_2746 -1.0 -3.8 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
Pf6N2E2_3611 -1.0 -1.3 HflC protein compare
Pf6N2E2_291 -1.0 -4.3 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_1072 -1.0 -4.8 LmbE-like protein compare
Pf6N2E2_3834 -1.0 -4.0 Lipoprotein NlpD compare
Pf6N2E2_5667 -1.0 -3.7 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_1294 -1.0 -2.7 Carbon starvation protein A compare
Pf6N2E2_5262 -1.0 -2.1 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf6N2E2_2578 -1.0 -1.0 Flagellar biosynthesis protein FliR compare
Pf6N2E2_5593 -1.0 -3.0 Leucyl-tRNA synthetase compare
Pf6N2E2_1705 -1.0 -1.3 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_3872 -1.0 -2.5 hypothetical protein compare
Pf6N2E2_2887 -1.0 -3.2 Aldose 1-epimerase compare
Pf6N2E2_4537 -1.0 -1.7 Type III secretion inner membrane protein (YscS,homologous to flagellar export components) compare
Pf6N2E2_4508 -0.9 -3.8 Transcriptional regulator, TetR family compare
Pf6N2E2_5226 -0.9 -1.4 FIG00955589: hypothetical protein compare
Pf6N2E2_5524 -0.9 -5.6 Outer membrane porin, OprD family compare
Pf6N2E2_3114 -0.9 -4.5 Transcriptional regulator, TetR family compare
Pf6N2E2_2041 -0.9 -3.2 D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) compare


Specific Phenotypes

For 14 genes in this experiment

For nitrogen source Urea in Pseudomonas fluorescens FW300-N2E2

For nitrogen source Urea across organisms