Experiment set8IT028 for Paraburkholderia bryophila 376MFSha3.1

Suberic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Suberic acid (10 mM)
Culturing: Burk376_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 0.5 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 5 genes in this experiment

For carbon source Suberic acid in Paraburkholderia bryophila 376MFSha3.1

For carbon source Suberic acid across organisms

SEED Subsystems

Subsystem	#Specific
Protocatechuate branch of beta-ketoadipate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism
• Other glycan degradation
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Benzoate degradation via CoA ligation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitin degradation II (Vibrio)	6	2	2
chitin degradation III (Serratia)	7	2	1
peptidoglycan recycling II	10	5	1
peptidoglycan recycling I	14	10	1