Experiment set8IT026 for Marinobacter adhaerens HP15

Compare to:

marine broth with Choline chloride 358.1 mM

Group: stress
Media: marine_broth_2216 + Choline chloride (358.1 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 934 E1,E2

Specific Phenotypes

For 24 genes in this experiment

For stress Choline chloride in Marinobacter adhaerens HP15

For stress Choline chloride across organisms

SEED Subsystems

Subsystem #Specific
Phosphate metabolism 3
Type IV pilus 3
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 2
Choline and Betaine Uptake and Betaine Biosynthesis 1
Conserved gene cluster associated with Met-tRNA formyltransferase 1
Ectoine biosynthesis and regulation 1
Fatty Acid Biosynthesis FASII 1
Heat shock dnaK gene cluster extended 1
Lipid A-Ara4N pathway ( Polymyxin resistance ) 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Teichuronic acid biosynthesis 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-glucuronate biosynthesis (from UDP-glucose) 1 1 1
UDP-α-D-xylose biosynthesis 2 2 1
phospholipid remodeling (phosphatidate, yeast) 2 1 1
palmitoleate biosynthesis III (cyanobacteria) 2 1 1
L-homoserine biosynthesis 3 3 1
oleate biosynthesis III (cyanobacteria) 3 2 1
cellulose degradation II (fungi) 3 1 1
gondoate biosynthesis (anaerobic) 4 4 1
CDP-diacylglycerol biosynthesis II 4 4 1
CDP-diacylglycerol biosynthesis I 4 4 1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 9 3
dipicolinate biosynthesis 4 3 1
fatty acid biosynthesis initiation (mitochondria) 4 3 1
spermidine biosynthesis II 4 2 1
L-methionine biosynthesis IV 4 2 1
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 9 2
oleate biosynthesis IV (anaerobic) 14 13 3
palmitate biosynthesis III 29 21 6
tRNA processing 10 10 2
fatty acid elongation -- saturated 5 5 1
ectoine biosynthesis 5 5 1
superpathway of fatty acid biosynthesis initiation 5 5 1
tetradecanoate biosynthesis (mitochondria) 25 17 5
CDP-diacylglycerol biosynthesis III 5 3 1
phosphatidate biosynthesis (yeast) 5 3 1
palmitate biosynthesis II (type II fatty acid synthase) 31 29 6
superpathway of fatty acid biosynthesis I (E. coli) 16 16 3
8-amino-7-oxononanoate biosynthesis I 11 9 2
superpathway of fatty acids biosynthesis (E. coli) 53 51 9
phosphatidylglycerol biosynthesis I 6 6 1
phosphatidylglycerol biosynthesis II 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
(5Z)-dodecenoate biosynthesis I 6 6 1
superpathway of phospholipid biosynthesis III (E. coli) 12 11 2
(5Z)-dodecenoate biosynthesis II 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
stearate biosynthesis IV 6 4 1
norspermidine biosynthesis 6 3 1
superpathway of stearidonate biosynthesis (cyanobacteria) 6 2 1
palmitoyl ethanolamide biosynthesis 6 2 1
superpathway of fatty acid biosynthesis II (plant) 43 38 7
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 20 3
L-lysine biosynthesis III 7 6 1
L-lysine biosynthesis VI 7 6 1
3-dehydroquinate biosynthesis II (archaea) 7 3 1
cremeomycin biosynthesis 7 2 1
stigma estolide biosynthesis 7 2 1
diacylglycerol and triacylglycerol biosynthesis 7 2 1
biotin biosynthesis I 15 13 2
superpathway of L-homoserine and L-methionine biosynthesis 8 6 1
superpathway of polyamine biosynthesis III 8 3 1
anandamide biosynthesis II 8 2 1
grixazone biosynthesis 8 2 1
streptorubin B biosynthesis 34 20 4
L-lysine biosynthesis I 9 9 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
L-lysine biosynthesis II 9 6 1
UDP-sugars interconversion 9 3 1
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
colanic acid building blocks biosynthesis 11 8 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
chorismate biosynthesis II (archaea) 12 8 1
anandamide biosynthesis I 12 4 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of cardiolipin biosynthesis (bacteria) 13 10 1
superpathway of phospholipid biosynthesis II (plants) 28 10 2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
plasmalogen biosynthesis I (aerobic) 16 2 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 23 1
platensimycin biosynthesis 26 7 1
mupirocin biosynthesis 26 2 1
mycolate biosynthesis 205 20 4
superpathway of mycolate biosynthesis 239 21 4