Experiment set8IT026 for Paraburkholderia bryophila 376MFSha3.1

Pimelic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Pimelic acid (10 mM)
Culturing: Burk376_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 1.4 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 4 genes in this experiment

For carbon source Pimelic acid in Paraburkholderia bryophila 376MFSha3.1

For carbon source Pimelic acid across organisms

SEED Subsystems

Subsystem	#Specific
Protocatechuate branch of beta-ketoadipate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism
• Benzoate degradation via CoA ligation