Experiment set8IT025 for Paraburkholderia graminis OAS925

Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days

Group: in planta
Media: MS_Basal_Salts_Robin_0.5x + Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days
Culturing: Burkholderia_OAS925_ML2, pot
By: Robin on 7/2/21
Media components: 825 mg/L Ammonium Nitrate, 3.1 mg/L Boric Acid, 166.1 mg/L Calcium chloride, 0.0125 mg/L Cobalt chloride hexahydrate, 0.0125 mg/L Copper (II) sulfate pentahydrate, 18.63 mg/L EDTA (disodium salt), 13.9 mg/L Iron (II) sulfate heptahydrate, 90.35 mg/L Magnesium sulfate, 8.45 mg/L Manganese sulfate, 0.415 mg/L Potassium iodide, 950 mg/L Potassium nitrate, 625 uM Potassium phosphate monobasic, 4.3 mg/L Zinc sulfate heptahydrate

Specific Phenotypes

For 24 genes in this experiment

For in planta Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days in Paraburkholderia graminis OAS925

For in planta Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial Chemotaxis	3
Aromatic amino acid degradation	1
Bacterial hemoglobins	1
Fructose utilization	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
NAD and NADP cofactor biosynthesis global	1
Orphan regulatory proteins	1
Polyamine Metabolism	1
Threonine and Homoserine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Alanine and aspartate metabolism
• Cysteine metabolism
• Arginine and proline metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Novobiocin biosynthesis
• Glyoxylate and dicarboxylate metabolism
• Carbon fixation in photosynthetic organisms
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-aspartate biosynthesis	1	1	1
3-(4-hydroxyphenyl)pyruvate biosynthesis	1	1	1
L-aspartate degradation I	1	1	1
malate/L-aspartate shuttle pathway	2	2	1
L-tryptophan degradation IV (via indole-3-lactate)	2	1	1
L-tyrosine degradation II	2	1	1
atromentin biosynthesis	2	1	1
L-glutamate degradation II	2	1	1
L-tryptophan degradation I (via anthranilate)	3	3	1
L-tyrosine biosynthesis I	3	3	1
L-phenylalanine biosynthesis I	3	3	1
L-asparagine degradation III (mammalian)	3	2	1
sulfolactate degradation III	3	2	1
(R)-cysteate degradation	3	1	1
L-tyrosine degradation IV (to 4-methylphenol)	3	1	1
indole-3-acetate biosynthesis VI (bacteria)	3	1	1
L-phenylalanine degradation II (anaerobic)	3	1	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	2	1
L-phenylalanine degradation III	4	2	1
L-tyrosine degradation III	4	2	1
dimethylsulfoniopropanoate biosynthesis III (algae and phytoplankton)	4	1	1
L-tryptophan degradation VIII (to tryptophol)	4	1	1
L-tyrosine degradation I	5	5	1
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde	5	3	1
trans-4-hydroxy-L-proline degradation I	5	3	1
3-hydroxy-4-methyl-anthranilate biosynthesis II	5	3	1
superpathway of plastoquinol biosynthesis	5	2	1
L-phenylalanine degradation VI (reductive Stickland reaction)	5	1	1
L-tryptophan degradation XIII (reductive Stickland reaction)	5	1	1
L-tyrosine degradation V (reductive Stickland reaction)	5	1	1
4-hydroxybenzoate biosynthesis I (eukaryotes)	5	1	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	7	2
superpathway of L-threonine biosynthesis	6	6	1
TCA cycle VIII (Chlamydia)	6	5	1
superpathway of sulfolactate degradation	6	3	1
3-hydroxy-4-methyl-anthranilate biosynthesis I	6	2	1
coenzyme M biosynthesis II	6	1	1
anaerobic energy metabolism (invertebrates, cytosol)	7	7	1
ethene biosynthesis III (microbes)	7	6	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	8	2
L-homomethionine biosynthesis	7	2	1
3-hydroxyquinaldate biosynthesis	8	2	1
Salmonella enterica serotype O:8 O antigen biosynthesis	8	2	1
superpathway of aromatic amino acid biosynthesis	18	18	2
NAD de novo biosynthesis II (from tryptophan)	9	7	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	6	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	3	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of L-tyrosine biosynthesis	10	10	1
quinoxaline-2-carboxylate biosynthesis	10	4	1
rosmarinic acid biosynthesis I	10	2	1
(S)-reticuline biosynthesis I	11	1	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	11	1
L-tryptophan degradation XII (Geobacillus)	12	4	1
indole-3-acetate biosynthesis II	12	4	1
L-tryptophan degradation IX	12	4	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of NAD biosynthesis in eukaryotes	14	8	1
superpathway of rosmarinic acid biosynthesis	14	2	1
L-tryptophan degradation III (eukaryotic)	15	6	1
superpathway of anaerobic energy metabolism (invertebrates)	17	12	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	15	1
L-tryptophan degradation XI (mammalian, via kynurenine)	23	7	1
aspartate superpathway	25	22	1
phosalacine biosynthesis	25	6	1
phosphinothricin tripeptide biosynthesis	25	6	1
anaerobic aromatic compound degradation (Thauera aromatica)	27	5	1
superpathway of chorismate metabolism	59	42	2
superpathway of aromatic compound degradation via 3-oxoadipate	35	19	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	13	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	23	1