Experiment set8IT020 for Desulfovibrio vulgaris Miyazaki F

Compare to:

MoLS4 minimal media with D,L-Cysteine

Group: nutrient
Media: MoLS4 + D,L-cysteine (1 mM), pH=7.2
Culturing: Miya_ML3_a, hungate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 20-Jun-16
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 2 genes in this experiment

For nutrient D,L-cysteine in Desulfovibrio vulgaris Miyazaki F

For nutrient D,L-cysteine across organisms

SEED Subsystems

Subsystem #Specific
De Novo Pyrimidine Synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) 4 2 1
photosynthesis light reactions 4 1 1
UMP biosynthesis II 6 6 1
superpathway of photosynthetic hydrogen production 6 2 1
oxygenic photosynthesis 17 10 1
ethene biosynthesis V (engineered) 25 16 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 13 1
1-butanol autotrophic biosynthesis (engineered) 27 14 1