Experiment set8IT014 for Desulfovibrio vulgaris Miyazaki F

MoLS4 minimal media with L-Histidine

Group: nutrient
Media: MoLS4 + L-Histidine (1 mM), pH=7.2
Culturing: Miya_ML3_a, hungate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 20-Jun-16
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 1 genes in this experiment

For nutrient L-Histidine in Desulfovibrio vulgaris Miyazaki F

For nutrient L-Histidine across organisms

SEED Subsystems

Subsystem	#Specific
One-carbon metabolism by tetrahydropterines	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formaldehyde oxidation VII (THF pathway)	4	4	1
folate transformations I	13	7	1
purine nucleobases degradation I (anaerobic)	15	5	1
purine nucleobases degradation II (anaerobic)	24	16	1