Experiment set8IT012 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Isoleucine nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4597 -6.5 -4.5 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_71 -6.5 -4.5 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4826 -6.3 -6.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3842 -6.2 -4.3 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3248 -6.2 -6.0 Probable transmembrane protein compare
Pf6N2E2_64 -6.1 -4.2 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_77 -6.0 -4.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4596 -5.9 -7.0 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3783 -5.8 -5.6 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4825 -5.8 -6.8 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3630 -5.6 -6.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5006 -5.6 -6.7 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_3253 -5.6 -5.5 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_3782 -5.6 -3.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_2258 -5.6 -9.3 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_5176 -5.6 -6.6 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_4047 -5.5 -3.8 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_5175 -5.3 -13.4 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_4279 -5.3 -12.9 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_5177 -5.2 -12.5 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3839 -5.2 -3.6 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_4071 -5.1 -5.8 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4048 -5.1 -8.5 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_477 -5.0 -6.8 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_5150 -4.9 -5.9 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_3940 -4.9 -12.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2885 -4.9 -3.4 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_3610 -4.9 -5.6 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_3932 -4.9 -6.2 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3170 -4.8 -15.9 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_4636 -4.8 -10.5 periplasmic binding protein, putative compare
Pf6N2E2_4453 -4.6 -19.0 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_2588 -4.6 -5.2 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_3264 -4.5 -4.4 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_6064 -4.5 -7.4 2-methylaconitate isomerase compare
Pf6N2E2_4564 -4.4 -8.6 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_3252 -4.4 -9.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3826 -4.4 -7.2 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3841 -4.3 -5.9 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4278 -4.3 -12.7 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_4187 -4.2 -2.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_5160 -4.1 -2.3 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_66 -4.1 -9.7 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_2586 -4.1 -2.8 Hpt domain protein compare
Pf6N2E2_4759 -4.0 -15.9 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_3072 -4.0 -13.6 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_5723 -3.9 -14.8 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_4072 -3.9 -4.6 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_50 -3.9 -10.4 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5520 -3.8 -9.8 Sensory box histidine kinase compare
Pf6N2E2_2073 -3.8 -9.4 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3661 -3.8 -6.2 hypothetical protein compare
Pf6N2E2_5030 -3.7 -8.4 Transcriptional regulator, AsnC family compare
Pf6N2E2_2863 -3.7 -10.2 Gluconate permease compare
Pf6N2E2_3825 -3.7 -5.6 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_4372 -3.6 -6.9 FIG00460773: hypothetical protein compare
Pf6N2E2_3251 -3.4 -12.7 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_3885 -3.4 -4.2 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_6063 -3.4 -10.1 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_5275 -3.4 -7.2 putative membrane protein conserved
Pf6N2E2_658 -3.4 -12.0 sensor histidine kinase compare
Pf6N2E2_2074 -3.3 -1.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_1147 -3.3 -3.4 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_4277 -3.2 -9.7 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_6140 -3.1 -1.2 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_4276 -3.1 -6.6 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_5153 -3.1 -8.8 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_3460 -3.0 -2.0 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_4638 -3.0 -4.1 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_3282 -3.0 -2.0 FIG005080: Possible exported protein compare
Pf6N2E2_2852 -2.9 -11.9 GGDEF domain/EAL domain protein compare
Pf6N2E2_5989 -2.9 -13.4 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) conserved
Pf6N2E2_5156 -2.9 -3.9 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4579 -2.9 -3.6 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_5728 -2.8 -5.2 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_3198 -2.8 -2.6 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_5328 -2.6 -6.5 FIG00958649: hypothetical protein compare
Pf6N2E2_5527 -2.6 -2.9 Cold shock protein CspC compare
Pf6N2E2_2218 -2.6 -5.7 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_5579 -2.4 -5.9 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_1056 -2.4 -1.2 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_3060 -2.4 -3.3 Outer membrane protein H precursor compare
Pf6N2E2_3984 -2.4 -10.3 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_4810 -2.4 -6.2 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_2756 -2.4 -8.7 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_2899 -2.4 -6.0 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_4618 -2.3 -4.9 Chemotaxis signal transduction protein compare
Pf6N2E2_2887 -2.3 -4.5 Aldose 1-epimerase compare
Pf6N2E2_3480 -2.3 -2.5 Transcriptional regulator, ArsR family compare
Pf6N2E2_2274 -2.3 -6.9 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_3586 -2.3 -7.5 NAD synthetase (EC 6.3.1.5) compare
Pf6N2E2_5494 -2.2 -4.0 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_3062 -2.2 -4.6 Membrane-associated zinc metalloprotease compare
Pf6N2E2_2864 -2.2 -2.5 Gluconokinase (EC 2.7.1.12) compare
Pf6N2E2_4365 -2.2 -5.2 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_481 -2.2 -8.3 branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) conserved
Pf6N2E2_482 -2.2 -4.3 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf6N2E2_2217 -2.1 -5.5 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_5219 -2.1 -9.2 FIG140336: TPR domain protein compare
Pf6N2E2_4009 -2.1 -9.7 GGDEF domain protein compare
Pf6N2E2_178 -2.1 -1.9 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_2706 -2.1 -2.9 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf6N2E2_2707 -2.1 -5.0 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_1932 -2.1 -5.3 Transcriptional regulator, IclR family compare
Pf6N2E2_4764 -2.0 -7.2 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_2465 -2.0 -6.5 Cys regulon transcriptional activator CysB compare
Pf6N2E2_4352 -2.0 -3.4 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_4364 -2.0 -2.9 Protein YicC compare
Pf6N2E2_5242 -1.9 -2.0 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_2442 -1.9 -9.9 sensor histidine kinase/response regulator compare
Pf6N2E2_2219 -1.9 -2.3 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_4362 -1.9 -2.9 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_3521 -1.9 -6.3 Sensor histidine kinase/response regulator compare
Pf6N2E2_1138 -1.8 -5.2 Transcriptional regulator, AraC family conserved
Pf6N2E2_2340 -1.8 -4.5 Transcriptional regulator, GntR family compare
Pf6N2E2_2897 -1.8 -9.2 Glucokinase (EC 2.7.1.2) compare
Pf6N2E2_63 -1.8 -2.4 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_4615 -1.8 -2.3 type IV pili signal transduction protein PilI compare
Pf6N2E2_5613 -1.8 -3.3 FIG00953674: hypothetical protein compare
Pf6N2E2_5045 -1.8 -11.0 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_4803 -1.8 -1.2 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_4614 -1.7 -4.4 twitching motility protein PilH compare
Pf6N2E2_4416 -1.7 -3.3 Tyrosine recombinase XerC compare
Pf6N2E2_149 -1.7 -7.6 Inner membrane component of tripartite multidrug resistance system compare
Pf6N2E2_1146 -1.7 -6.1 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) conserved
Pf6N2E2_5157 -1.7 -8.1 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5331 -1.7 -5.4 ATPase, AFG1 family compare
Pf6N2E2_3283 -1.7 -5.8 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_2186 -1.7 -7.0 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_480 -1.7 -9.5 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) conserved
Pf6N2E2_2447 -1.7 -5.1 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_2708 -1.6 -4.0 Lactam utilization protein LamB compare
Pf6N2E2_6061 -1.6 -5.4 Methylisocitrate lyase (EC 4.1.3.30) (from data) compare
Pf6N2E2_4413 -1.6 -7.5 FIG004064: hypothetical protein compare
Pf6N2E2_5001 -1.6 -1.5 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_942 -1.6 -2.4 Mll6465 protein compare
Pf6N2E2_3875 -1.6 -1.8 FIG00953324: hypothetical protein compare
Pf6N2E2_4631 -1.6 -7.9 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_4763 -1.6 -8.7 Biotin synthesis protein BioC compare
Pf6N2E2_4074 -1.6 -6.4 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_3835 -1.6 -2.7 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_613 -1.6 -1.8 lipoprotein, putative compare
Pf6N2E2_4399 -1.5 -6.9 GGDEF domain/EAL domain protein compare
Pf6N2E2_4392 -1.5 -2.6 Translation initiation inhibitor compare
Pf6N2E2_3461 -1.5 -1.5 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_51 -1.5 -5.3 hypothetical protein compare
Pf6N2E2_4611 -1.5 -4.4 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_2891 -1.5 -9.0 Glucose ABC transport system, inner membrane component 1 compare
Pf6N2E2_148 -1.5 -7.9 Membrane fusion component of tripartite multidrug resistance system compare
Pf6N2E2_5158 -1.5 -3.6 FIG00954153: hypothetical protein compare
Pf6N2E2_2530 -1.5 -8.2 Sensory box/GGDEF family protein compare
Pf6N2E2_5420 -1.5 -5.3 Cytosol aminopeptidase PepA (EC 3.4.11.1) compare
Pf6N2E2_4388 -1.5 -8.1 Gamma-glutamyl-putrescine oxidase (EC1.4.3.-) compare
Pf6N2E2_4417 -1.4 -2.8 Protein of unknown function DUF484 compare
Pf6N2E2_2753 -1.4 -2.7 HtrA protease/chaperone protein compare
Pf6N2E2_3849 -1.4 -2.4 FIG00954739: hypothetical protein compare
Pf6N2E2_3516 -1.4 -2.1 Probable transmembrane protein compare
Pf6N2E2_4617 -1.4 -9.0 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_3655 -1.4 -9.4 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_4761 -1.4 -8.4 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_2892 -1.3 -8.9 Glucose ABC transport system, periplasmic sugar-binding protein compare
Pf6N2E2_1043 -1.3 -4.8 Transcriptional regulator, IclR family compare
Pf6N2E2_828 -1.3 -5.0 Transcriptional regulator, TetR family compare
Pf6N2E2_3349 -1.3 -1.9 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_479 -1.3 -3.1 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) compare
Pf6N2E2_2281 -1.3 -2.6 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_4762 -1.3 -3.4 Biotin synthesis protein BioH compare
Pf6N2E2_3677 -1.3 -9.0 Sensory box/GGDEF family protein compare
Pf6N2E2_4059 -1.3 -1.3 DNA-binding protein HU-alpha compare
Pf6N2E2_2574 -1.3 -1.2 Flagellar synthesis regulator FleN compare
Pf6N2E2_2895 -1.3 -7.1 Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster compare
Pf6N2E2_5425 -1.3 -1.2 hypothetical protein compare
Pf6N2E2_2526 -1.3 -4.0 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_147 -1.3 -7.5 Outer membrane component of tripartite multidrug resistance system compare
Pf6N2E2_5679 -1.3 -3.1 hypothetical protein compare
Pf6N2E2_5252 -1.3 -1.1 FIG00956396: hypothetical protein compare
Pf6N2E2_2795 -1.2 -2.0 putative exported protein compare
Pf6N2E2_5499 -1.2 -1.3 hypothetical protein compare
Pf6N2E2_2201 -1.2 -3.3 Transmembrane regulator protein PrtR compare
Pf6N2E2_2896 -1.2 -4.1 DNA-binding response regulator GltR, controls specific porins for the entry of glucose compare
Pf6N2E2_5780 -1.2 -6.3 Periplasmic protease compare
Pf6N2E2_4097 -1.2 -6.3 FIG00953365: hypothetical protein compare
Pf6N2E2_6060 -1.2 -3.0 Propionate catabolism operon transcriptional regulator of GntR family [predicted] compare
Pf6N2E2_1606 -1.2 -3.9 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_4710 -1.2 -1.7 hypothetical protein compare
Pf6N2E2_2473 -1.2 -1.2 Major outer membrane lipoprotein I compare
Pf6N2E2_1694 -1.2 -1.3 FMN reductase (EC 1.5.1.29) compare
Pf6N2E2_623 -1.1 -1.5 FIG00954674: hypothetical protein compare
Pf6N2E2_5103 -1.1 -0.8 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_2888 -1.1 -7.9 Glucose-selective porin OprB compare
Pf6N2E2_4412 -1.1 -6.7 Ammonium transporter compare
Pf6N2E2_3696 -1.1 -1.0 hypothetical protein compare
Pf6N2E2_5173 -1.1 -3.9 Hypothetical protein YqcC (clustered with tRNA pseudouridine synthase C) compare
Pf6N2E2_3277 -1.1 -2.0 FagA protein compare
Pf6N2E2_2248 -1.1 -7.5 Aspartate racemase compare
Pf6N2E2_2189 -1.1 -3.9 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_3666 -1.1 -2.1 InaA protein compare
Pf6N2E2_2890 -1.1 -5.3 Glucose ABC transport system, inner membrane component 2 compare
Pf6N2E2_1676 -1.1 -2.3 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_5188 -1.1 -4.3 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare


Specific Phenotypes

For 21 genes in this experiment

For nitrogen source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Isoleucine across organisms