Experiment set8IT012 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Isoleucine nitrogen source

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5155 +5.9 12.5 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_4369 +5.1 15.0 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_3271 +3.1 12.3 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3942 +2.9 16.6 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_146 +2.1 7.3 Transcriptional regulator, MarR family compare
Pf6N2E2_2406 +1.8 10.8 Periplasmic binding protein compare
Pf6N2E2_3941 +1.7 3.9 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3978 +1.7 13.7 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_3869 +1.7 11.1 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_6001 +1.7 10.2 Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_3979 +1.6 12.1 Type I secretion system ATPase, LssB family LapB compare
Pf6N2E2_2921 +1.5 11.3 ABC transporter for L-leucine/L-phenylalanine/D-alanine, substrate-binding component LivK (from data) compare
Pf6N2E2_2925 +1.5 5.5 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 1 LivG (from data) conserved
Pf6N2E2_2924 +1.5 11.0 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, permease component 2 LivM (from data) conserved
Pf6N2E2_3685 +1.5 10.9 Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) compare
Pf6N2E2_5720 +1.4 9.5 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_5874 +1.4 11.9 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf6N2E2_5403 +1.4 9.1 ABC transporter for D-Alanine, permease component 2 (from data) conserved
Pf6N2E2_3908 +1.4 7.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2926 +1.4 4.3 ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) conserved
Pf6N2E2_3134 +1.3 1.6 hypothetical protein compare
Pf6N2E2_3981 +1.3 12.1 T1SS secreted agglutinin RTX compare
Pf6N2E2_2923 +1.3 9.6 ABC transporter for L-leucine/L-phenylalanine/D-alanine, permease component 1 LivH (from data) conserved
Pf6N2E2_3969 +1.3 1.3 Membrane-fusion protein compare
Pf6N2E2_2022 +1.3 6.8 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_5084 +1.3 9.2 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_1774 +1.2 1.8 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_2587 +1.2 8.5 Serine phosphatase RsbU, regulator of sigma subunit compare
Pf6N2E2_1083 +1.2 2.4 hypothetical protein compare
Pf6N2E2_1617 +1.2 1.4 COG2199: FOG: GGDEF domain compare
Pf6N2E2_5404 +1.1 6.8 ABC transporter for D-Alanine, permease component 1 (from data) conserved
Pf6N2E2_3791 +1.1 7.5 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_1981 +1.1 3.3 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_5346 +1.1 2.6 rRNA small subunit methyltransferase I compare
Pf6N2E2_2284 +1.1 2.5 Cell division inhibitor-related protein compare
Pf6N2E2_923 +1.1 2.8 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_5261 +1.1 1.7 FIG00956983: hypothetical protein compare
Pf6N2E2_3792 +1.1 7.0 Glucans biosynthesis protein G precursor compare
Pf6N2E2_2441 +1.1 4.4 putative ATP/GTP-binding protein compare
Pf6N2E2_1824 +1.0 1.3 hypothetical protein compare
Pf6N2E2_3475 +1.0 2.8 hypothetical protein compare
Pf6N2E2_2922 +1.0 2.2 hypothetical protein compare
Pf6N2E2_2755 +1.0 2.1 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_5872 +1.0 5.9 Sensor histidine kinase/response regulator compare
Pf6N2E2_4418 +1.0 1.1 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_2121 +1.0 2.5 hypothetical protein compare
Pf6N2E2_3752 +1.0 8.6 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_605 +1.0 2.1 FIG002465: BNR repeat protein compare
Pf6N2E2_445 +1.0 4.8 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_3980 +1.0 7.5 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_5025 +1.0 9.1 Sensor histidine kinase compare
Pf6N2E2_5877 +1.0 2.4 Phosphohistidine phosphatase SixA compare
Pf6N2E2_5329 +1.0 4.1 Alpha/beta hydrolase compare
Pf6N2E2_3830 +1.0 3.4 Glutaredoxin 3 (Grx3) compare
Pf6N2E2_3569 +1.0 2.2 hypothetical protein compare
Pf6N2E2_5865 +0.9 7.0 Chaperone protein HtpG compare
Pf6N2E2_1340 +0.9 2.8 major pilin protein fimA compare
Pf6N2E2_2625 +0.9 3.5 Flagellar P-ring protein FlgI compare
Pf6N2E2_1176 +0.9 2.5 Mll2356 protein compare
Pf6N2E2_5229 +0.9 4.1 Transcriptional regulator, MerR family, associated with photolyase compare
Pf6N2E2_2675 +0.9 1.0 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_4640 +0.9 2.0 hypothetical protein compare
Pf6N2E2_3871 +0.9 6.7 FIG00553872: hypothetical protein compare
Pf6N2E2_5522 +0.9 2.3 FIG001943: hypothetical protein YajQ compare
Pf6N2E2_5573 +0.9 3.5 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_5405 +0.9 4.8 ABC transporter for D-Alanine, ATPase component (from data) compare
Pf6N2E2_4057 +0.9 1.2 lipoprotein, putative compare
Pf6N2E2_2225 +0.9 2.7 FIG00953489: hypothetical protein compare
Pf6N2E2_3751 +0.9 6.5 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_3338 +0.9 1.9 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_648 +0.9 1.8 General secretion pathway protein K compare
Pf6N2E2_2822 +0.9 5.4 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_3325 +0.8 2.0 ABC transporter, ATP-binding protein compare
Pf6N2E2_4747 +0.8 2.5 Transmembrane sensor compare
Pf6N2E2_4889 +0.8 4.4 Putative transport protein compare
Pf6N2E2_3455 +0.8 2.5 Integral membrane protein YfiB compare
Pf6N2E2_4422 +0.8 2.2 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_2578 +0.8 1.6 Flagellar biosynthesis protein FliR compare
Pf6N2E2_3122 +0.8 2.1 Chemotaxis signal transduction protein compare
Pf6N2E2_5873 +0.8 3.4 FIG00953467: hypothetical protein compare
Pf6N2E2_5207 +0.8 1.7 hypothetical protein compare
Pf6N2E2_3625 +0.8 6.2 Flagellar motor rotation protein MotA compare
Pf6N2E2_597 +0.8 3.9 FIG00953564: hypothetical protein compare
Pf6N2E2_5014 +0.8 1.4 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_3899 +0.8 4.4 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_2438 +0.8 1.7 hypothetical protein compare
Pf6N2E2_2597 +0.8 5.6 Flagellar regulatory protein FleQ compare
Pf6N2E2_4540 +0.8 2.7 hypothetical protein compare
Pf6N2E2_2681 +0.8 1.8 3-phosphoglycerate kinase compare
Pf6N2E2_5629 +0.8 2.5 Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_4381 +0.8 3.6 hypothetical protein compare
Pf6N2E2_1671 +0.8 3.2 Transcriptional regulator, TetR family compare
Pf6N2E2_987 +0.8 1.9 hypothetical protein compare
Pf6N2E2_2178 +0.8 4.5 FIG00960144: hypothetical protein compare
Pf6N2E2_1299 +0.8 2.3 Transcriptional regulator, TetR family compare
Pf6N2E2_2300 +0.8 3.2 LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM compare
Pf6N2E2_3761 +0.8 6.5 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_3192 +0.8 3.3 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_4591 +0.8 2.8 tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) compare
Pf6N2E2_4029 +0.8 4.3 Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-) compare
Pf6N2E2_2111 +0.8 2.4 Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit A (from data) compare
Pf6N2E2_4541 +0.8 1.3 hypothetical protein compare
Pf6N2E2_1014 +0.8 1.7 Nitrate/nitrite transporter compare
Pf6N2E2_5491 +0.7 4.9 FOG: GGDEF domain compare
Pf6N2E2_2487 +0.7 1.0 FIG00956090: hypothetical protein compare
Pf6N2E2_1743 +0.7 2.5 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) compare
Pf6N2E2_2240 +0.7 3.7 Ribonuclease T2 family protein compare
Pf6N2E2_4612 +0.7 3.1 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_5260 +0.7 3.6 Phosphate acetyltransferase (EC 2.3.1.8) compare
Pf6N2E2_1567 +0.7 2.7 FIG00964846: hypothetical protein compare
Pf6N2E2_2436 +0.7 2.7 DNA-binding response regulator, LuxR family compare
Pf6N2E2_5230 +0.7 4.7 COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA compare
Pf6N2E2_1336 +0.7 2.0 AraC family transcription regulator compare
Pf6N2E2_3179 +0.7 1.6 FIG021952: putative membrane protein compare
Pf6N2E2_54 +0.7 1.7 Transcriptional regulator, TetR family compare
Pf6N2E2_218 +0.7 2.4 hypothetical protein compare
Pf6N2E2_282 +0.7 1.5 GNAT family acetyltransferase YjcF compare
Pf6N2E2_204 +0.7 1.4 hypothetical protein compare
Pf6N2E2_3183 +0.7 2.4 Believed to be involved in assembly of Fe-S clusters compare
Pf6N2E2_1522 +0.7 2.5 Alcohol dehydrogenase (EC 1.1.1.1) compare
Pf6N2E2_3870 +0.7 1.0 hypothetical protein compare
Pf6N2E2_3328 +0.7 1.1 transcriptional regulator compare
Pf6N2E2_2114 +0.7 1.8 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_3807 +0.7 2.5 Histidine utilization repressor compare
Pf6N2E2_3088 +0.7 1.5 hypothetical protein compare
Pf6N2E2_1165 +0.7 1.8 hypothetical protein compare
Pf6N2E2_3029 +0.7 2.4 Holin compare
Pf6N2E2_1319 +0.7 2.6 FIG074102: hypothetical protein compare
Pf6N2E2_3456 +0.7 3.8 Inner membrane protein YfiN compare
Pf6N2E2_4499 +0.7 1.9 protein of unknown function DUF86 compare
Pf6N2E2_1782 +0.7 1.1 STAS domain protein compare
Pf6N2E2_5251 +0.6 1.5 FIG00954698: hypothetical protein compare
Pf6N2E2_4984 +0.6 2.8 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_434 +0.6 1.5 hypothetical protein compare
Pf6N2E2_3847 +0.6 3.0 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_6059 +0.6 2.1 FIG008443: hypothetical protein compare
Pf6N2E2_6035 +0.6 2.4 alpha/beta hydrolase fold compare
Pf6N2E2_1679 +0.6 3.0 SUGAR TRANSPORTER compare
Pf6N2E2_3983 +0.6 3.5 Membrane bound c-di-GMP receptor LapD compare
Pf6N2E2_1797 +0.6 1.4 Transcriptional regulator compare
Pf6N2E2_1215 +0.6 1.6 probable membrane protein YPO2362 compare
Pf6N2E2_1506 +0.6 1.1 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_6113 +0.6 2.9 tRNA-Gly-CCC compare
Pf6N2E2_4171 +0.6 2.2 hypothetical protein compare
Pf6N2E2_2788 +0.6 1.0 Transcriptional regulator, AsnC family compare
Pf6N2E2_5923 +0.6 2.7 FIG00956241: hypothetical protein compare
Pf6N2E2_402 +0.6 2.3 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_1040 +0.6 1.1 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_1817 +0.6 2.0 hypothetical protein compare
Pf6N2E2_3827 +0.6 3.8 FIG00955429: hypothetical protein compare
Pf6N2E2_4336 +0.6 3.8 Uncharacterized protein ImpA compare
Pf6N2E2_2571 +0.6 3.3 Chemotaxis response - phosphatase CheZ compare
Pf6N2E2_2509 +0.6 1.2 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_1028 +0.6 2.4 Transcriptional regulator, LysR family compare
Pf6N2E2_1998 +0.6 2.8 FIG01212208: hypothetical protein compare
Pf6N2E2_5794 +0.6 1.4 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_3162 +0.6 2.1 hypothetical protein compare
Pf6N2E2_1353 +0.6 2.6 FIG002577: Putative lipoprotein precursor compare
Pf6N2E2_5505 +0.6 1.7 hypothetical protein compare
Pf6N2E2_2286 +0.6 1.3 FIG00953287: hypothetical protein compare
Pf6N2E2_746 +0.6 1.2 FIG00958097: hypothetical protein compare
Pf6N2E2_1022 +0.6 1.8 Butyryl-CoA dehydrogenase (EC 1.3.99.2) compare
Pf6N2E2_48 +0.6 1.4 FIG00955661: hypothetical protein compare
Pf6N2E2_3679 +0.6 3.0 Protein involved in catabolism of external DNA compare
Pf6N2E2_4030 +0.6 4.2 Biofilm PGA synthesis deacetylase PgaB (EC 3.-) compare
Pf6N2E2_1802 +0.6 2.2 polysaccharide deacetylase compare
Pf6N2E2_2750 +0.6 3.2 Putative permease PerM (= YfgO) compare
Pf6N2E2_5077 +0.6 1.9 hypothetical protein compare
Pf6N2E2_1809 +0.6 2.7 Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1) compare
Pf6N2E2_1678 +0.6 3.7 Major facilitator family transporter compare
Pf6N2E2_3788 +0.6 3.1 Methyl-accepting chemotaxis protein I (serine chemoreceptor protein) compare
Pf6N2E2_2592 +0.6 2.5 Flagellar motor switch protein FliG compare
Pf6N2E2_3868 +0.6 3.4 Transcriptional regulator, LysR family compare
Pf6N2E2_3713 +0.6 1.2 Acyl carrier protein compare
Pf6N2E2_3676 +0.6 4.3 Phosphodiesterase/alkaline phosphatase D-like compare
Pf6N2E2_6029 +0.6 1.5 hypothetical protein compare
Pf6N2E2_5758 +0.6 0.8 FIG00957314: hypothetical protein compare
Pf6N2E2_603 +0.6 1.9 FIG00953934: hypothetical protein compare
Pf6N2E2_841 +0.6 1.2 hypothetical protein compare
Pf6N2E2_4045 +0.6 1.5 FIG00953248: hypothetical protein compare
Pf6N2E2_2106 +0.6 0.7 hypothetical protein compare
Pf6N2E2_659 +0.6 1.1 Transcriptional regulatory protein ompR compare
Pf6N2E2_943 +0.6 1.7 Transcriptional regulator, AraC family compare
Pf6N2E2_6087 +0.6 2.0 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_1162 +0.6 1.1 Transmembrane amino acid efflux protein compare
Pf6N2E2_2288 +0.6 1.6 FIG00953423: hypothetical protein compare
Pf6N2E2_5020 +0.6 2.6 FIG00955339: hypothetical protein compare
Pf6N2E2_4052 +0.6 1.0 FIG00955481: hypothetical protein compare
Pf6N2E2_4841 +0.6 2.8 Biotin operon repressor / Biotin-protein ligase (EC 6.3.4.15) compare
Pf6N2E2_1674 +0.6 2.9 Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) compare
Pf6N2E2_5345 +0.5 2.8 LppC putative lipoprotein compare
Pf6N2E2_476 +0.5 1.7 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3690 +0.5 3.2 Na(+)/H(+) antiporter compare
Pf6N2E2_4031 +0.5 4.6 Biofilm PGA outer membrane secretin PgaA compare
Pf6N2E2_4673 +0.5 1.2 ATP-dependent 23S rRNA helicase DbpA compare
Pf6N2E2_1875 +0.5 1.7 Protoporphyrinogen oxidase (EC 1.3.3.4) compare
Pf6N2E2_5862 +0.5 1.3 Lipoprotein, putative compare
Pf6N2E2_3093 +0.5 2.2 Transcriptional regulator, TetR family compare
Pf6N2E2_464 +0.5 1.2 Sensory histidine kinase QseC compare
Pf6N2E2_3397 +0.5 1.2 Phage protein compare


Specific Phenotypes

For 21 genes in this experiment

For nitrogen source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Isoleucine across organisms