Experiment set8IT012 for Pseudomonas fluorescens FW300-N2E2

L-Isoleucine nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Isoleucine (20 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
Growth: about 4.1 generations
By: Mark on 1/21/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 21 genes in this experiment

For nitrogen source L-Isoleucine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Isoleucine across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter branched-chain amino acid (TC 3.A.1.4.1)	4
Isoleucine degradation	3
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	3
Valine degradation	3
Leucine Degradation and HMG-CoA Metabolism	2
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Butanol Biosynthesis	1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Folate Biosynthesis	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Methylcitrate cycle	1
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Propionate-CoA to Succinate Module	1
Queuosine-Archaeosine Biosynthesis	1
Threonine and Homoserine Biosynthesis	1
Tryptophan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Valine, leucine and isoleucine degradation
• Arginine and proline metabolism
• Biosynthesis of phenylpropanoids
• Fatty acid metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Cysteine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Novobiocin biosynthesis
• Propanoate metabolism
• Butanoate metabolism
• Carbon fixation in photosynthetic organisms
• Nicotinate and nicotinamide metabolism
• Folate biosynthesis
• Nitrogen metabolism
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamine degradation I	1	1	1
L-aspartate biosynthesis	1	1	1
3-(4-hydroxyphenyl)pyruvate biosynthesis	1	1	1
L-aspartate degradation I	1	1	1
L-glutamate degradation II	2	2	1
4-aminobenzoate biosynthesis I	2	2	1
L-glutamate biosynthesis I	2	2	1
atromentin biosynthesis	2	1	1
L-tryptophan degradation IV (via indole-3-lactate)	2	1	1
malate/L-aspartate shuttle pathway	2	1	1
L-tyrosine degradation II	2	1	1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA	3	3	1
ammonia assimilation cycle III	3	3	1
L-tyrosine biosynthesis I	3	3	1
L-phenylalanine biosynthesis I	3	3	1
L-asparagine degradation III (mammalian)	3	2	1
L-phenylalanine degradation II (anaerobic)	3	2	1
(R)-cysteate degradation	3	1	1
L-tyrosine degradation IV (to 4-methylphenol)	3	1	1
indole-3-acetate biosynthesis VI (bacteria)	3	1	1
sulfolactate degradation III	3	1	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
putrescine degradation II	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	3	1
L-phenylalanine degradation III	4	2	1
L-tyrosine degradation III	4	2	1
L-tryptophan degradation VIII (to tryptophol)	4	1	1
L-tyrosine degradation I	5	5	1
2-methylcitrate cycle I	5	5	1
NAD salvage pathway II (PNC IV cycle)	5	4	1
trans-4-hydroxy-L-proline degradation I	5	3	1
superpathway of plastoquinol biosynthesis	5	2	1
L-phenylalanine degradation VI (reductive Stickland reaction)	5	1	1
L-tryptophan degradation XIII (reductive Stickland reaction)	5	1	1
L-tyrosine degradation V (reductive Stickland reaction)	5	1	1
4-hydroxybenzoate biosynthesis I (eukaryotes)	5	1	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	6	2
superpathway of L-threonine biosynthesis	6	6	1
NAD de novo biosynthesis I	6	5	1
L-isoleucine degradation I	6	5	1
2-methylcitrate cycle II	6	5	1
NAD de novo biosynthesis IV (anaerobic)	6	5	1
TCA cycle VIII (Chlamydia)	6	4	1
pyruvate fermentation to butanol II (engineered)	6	4	1
NAD de novo biosynthesis III	6	4	1
propanoate fermentation to 2-methylbutanoate	6	4	1
superpathway of sulfolactate degradation	6	2	1
coenzyme M biosynthesis II	6	1	1
L-glutamate and L-glutamine biosynthesis	7	6	1
NAD salvage pathway I (PNC VI cycle)	7	5	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	9	2
anaerobic energy metabolism (invertebrates, cytosol)	7	4	1
superpathway of ornithine degradation	8	7	1
L-valine degradation I	8	6	1
L-citrulline biosynthesis	8	6	1
superpathway of aromatic amino acid biosynthesis	18	18	2
superpathway of L-methionine biosynthesis (transsulfuration)	9	7	1
valproate β-oxidation	9	7	1
L-phenylalanine degradation IV (mammalian, via side chain)	9	5	1
chloramphenicol biosynthesis	9	1	1
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
rosmarinic acid biosynthesis I	10	3	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	9	1
pyruvate fermentation to hexanol (engineered)	11	7	1
superpathway of candicidin biosynthesis	11	4	1
(S)-reticuline biosynthesis I	11	1	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
superpathway of L-citrulline metabolism	12	8	1
indole-3-acetate biosynthesis II	12	5	1
aspartate superpathway	25	22	2
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-arginine and L-ornithine degradation	13	11	1
2-methyl-branched fatty acid β-oxidation	14	11	1
superpathway of rosmarinic acid biosynthesis	14	4	1
superpathway of anaerobic energy metabolism (invertebrates)	17	8	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
superpathway of chorismate metabolism	59	43	3
1-butanol autotrophic biosynthesis (engineered)	27	20	1
anaerobic aromatic compound degradation (Thauera aromatica)	27	4	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	21	1