Experiment set8IT012 for Desulfovibrio vulgaris Hildenborough JW710

Compare to:

Pyruvate-Sulfite (60-20mM) with Molybdate 100uM

Group: stress
Media: Dv_base_medium + Sodium pyruvate (60 mM) + sodium sulfite (20 mM) + Sodium molybdate (100 uM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 3/3/2017
Media components: 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 25 genes in this experiment

For stress Sodium pyruvate in Desulfovibrio vulgaris Hildenborough JW710

For stress Sodium pyruvate across organisms

SEED Subsystems

Subsystem #Specific
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes 5
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 3
DNA structural proteins, bacterial 1
Flagellar motility 1
Flagellum 1
Nitrogen fixation 1
Protein chaperones 1
Ribosome biogenesis bacterial 1
Transport of Zinc 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: