Experiment set8IT007 for Bacteroides thetaiotaomicron VPI-5482
BHIS with Thioridazine hydrochloride 0.025 mM
Group: stressMedia: BHIS + Thioridazine hydrochloride (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Specific Phenotypes
For 7 genes in this experiment
For stress Thioridazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482
For stress Thioridazine hydrochloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Signal peptidase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Biosynthesis of alkaloids derived from shikimate pathway
- Glycolysis / Gluconeogenesis
- Citrate cycle (TCA cycle)
- Ubiquinone and menaquinone biosynthesis
- Glycine, serine and threonine metabolism
- Valine, leucine and isoleucine degradation
- Histidine metabolism
- Tyrosine metabolism
- Tryptophan metabolism
- Benzoxazinone biosynthesis
- Pyruvate metabolism
- Naphthalene and anthracene degradation
- Porphyrin and chlorophyll metabolism
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| phenylmercury acetate degradation | 2 | 1 | 1 |
| glycine biosynthesis II | 3 | 3 | 1 |
| glycine cleavage | 3 | 3 | 1 |
| 2-oxoglutarate decarboxylation to succinyl-CoA | 3 | 2 | 1 |
| pyruvate decarboxylation to acetyl CoA I | 3 | 2 | 1 |
| 2-oxoisovalerate decarboxylation to isobutanoyl-CoA | 3 | 2 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 17 | 1 |