Experiment set8IT005 for Pseudomonas fluorescens FW300-N2E2

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L-Glutamic acid monopotassium salt monohydrate nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4826 -6.3 -4.3 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3610 -6.2 -4.3 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4597 -6.1 -4.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_71 -6.1 -4.2 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3248 -5.8 -5.7 Probable transmembrane protein compare
Pf6N2E2_3932 -5.8 -6.9 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3783 -5.8 -4.0 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4825 -5.7 -5.5 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_64 -5.7 -3.9 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_77 -5.6 -3.8 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_5175 -5.3 -9.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3630 -5.2 -6.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3763 -5.2 -3.6 hypothetical protein compare
Pf6N2E2_3842 -5.2 -5.0 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3782 -5.2 -3.6 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_4596 -5.1 -8.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_4047 -5.1 -3.5 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4564 -5.0 -11.2 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_66 -4.8 -9.8 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_5177 -4.7 -11.0 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3252 -4.7 -7.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_5176 -4.6 -8.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3253 -4.4 -6.9 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_2073 -4.4 -6.1 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5014 -4.4 -2.0 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_4048 -4.4 -8.8 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3839 -4.4 -4.2 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_50 -4.3 -8.6 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5258 -4.3 -9.0 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_2465 -4.2 -7.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_3762 -4.1 -6.2 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_3940 -4.1 -11.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4762 -4.0 -4.5 Biotin synthesis protein BioH compare
Pf6N2E2_4759 -3.9 -15.6 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_5257 -3.9 -11.6 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_3841 -3.9 -5.3 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4206 -3.8 -2.6 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4761 -3.6 -11.6 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_4763 -3.6 -11.8 Biotin synthesis protein BioC compare
Pf6N2E2_3938 -3.4 -14.6 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_302 -3.4 -5.9 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_178 -3.3 -2.1 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_3251 -3.3 -10.5 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_4764 -3.1 -7.4 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_5527 -3.0 -2.8 Cold shock protein CspC compare
Pf6N2E2_2074 -3.0 -1.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_5579 -2.9 -7.9 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_4631 -2.9 -9.0 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_3349 -2.8 -3.8 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_5676 -2.8 -4.7 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_3460 -2.7 -1.8 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_2753 -2.7 -3.1 HtrA protease/chaperone protein compare
Pf6N2E2_3170 -2.7 -6.6 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_5316 -2.6 -2.5 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_4638 -2.6 -3.5 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_5728 -2.4 -3.7 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_2477 -2.4 -2.5 ABC transporter ATP-binding protein YvcR compare
Pf6N2E2_63 -2.4 -4.5 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_5130 -2.3 -2.2 hypothetical protein compare
Pf6N2E2_5950 -2.2 -3.0 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_4077 -2.1 -2.2 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_4416 -2.1 -2.7 Tyrosine recombinase XerC compare
Pf6N2E2_3261 -2.1 -4.1 hypothetical protein compare
Pf6N2E2_3984 -2.0 -9.3 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_5006 -2.0 -4.9 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_51 -1.9 -6.5 hypothetical protein compare
Pf6N2E2_2218 -1.9 -4.7 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_4009 -1.9 -7.8 GGDEF domain protein compare
Pf6N2E2_2277 -1.9 -2.4 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_122 -1.9 -2.7 Tyrosine-protein kinase Wzc (EC 2.7.10.2) compare
Pf6N2E2_5153 -1.8 -5.2 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_3908 -1.8 -5.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_4153 -1.8 -1.9 Sensory box histidine kinase compare
Pf6N2E2_3060 -1.8 -3.6 Outer membrane protein H precursor compare
Pf6N2E2_4276 -1.7 -4.1 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_4187 -1.7 -4.0 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_4797 -1.7 -1.6 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_4963 -1.7 -2.3 Homoserine dehydrogenase (EC 1.1.1.3) compare
Pf6N2E2_2440 -1.7 -1.4 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf6N2E2_5425 -1.7 -1.3 hypothetical protein compare
Pf6N2E2_623 -1.7 -1.8 FIG00954674: hypothetical protein compare
Pf6N2E2_5816 -1.7 -1.9 Smr domain protein compare
Pf6N2E2_4417 -1.7 -3.5 Protein of unknown function DUF484 compare
Pf6N2E2_5103 -1.6 -1.0 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_2588 -1.6 -2.7 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_3611 -1.6 -1.7 HflC protein compare
Pf6N2E2_2219 -1.5 -1.9 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_4454 -1.5 -3.8 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_5746 -1.5 -2.4 Cobalamin synthase compare
Pf6N2E2_2473 -1.5 -1.4 Major outer membrane lipoprotein I compare
Pf6N2E2_3535 -1.5 -4.9 Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) compare
Pf6N2E2_2312 -1.5 -1.6 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_4916 -1.4 -3.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_1870 -1.4 -2.4 hypothetical protein compare
Pf6N2E2_1656 -1.4 -2.9 hypothetical protein compare
Pf6N2E2_3351 -1.3 -3.4 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_3257 -1.3 -3.1 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_2885 -1.3 -4.8 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_3516 -1.3 -2.3 Probable transmembrane protein compare
Pf6N2E2_1893 -1.3 -2.1 hypothetical protein compare
Pf6N2E2_2519 -1.3 -1.1 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_1258 -1.3 -3.1 FIG00960119: hypothetical protein compare
Pf6N2E2_3264 -1.3 -1.4 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3258 -1.3 -3.7 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_3687 -1.3 -2.1 FIG00959437: hypothetical protein compare
Pf6N2E2_4362 -1.2 -1.7 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_3849 -1.2 -2.1 FIG00954739: hypothetical protein compare
Pf6N2E2_89 -1.2 -1.5 hypothetical protein compare
Pf6N2E2_2420 -1.2 -2.4 hypothetical protein compare
Pf6N2E2_5739 -1.2 -1.2 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family compare
Pf6N2E2_5156 -1.2 -2.2 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2510 -1.2 -2.3 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_199 -1.2 -3.0 hypothetical protein compare
Pf6N2E2_2397 -1.2 -2.9 Two-component system response regulator QseB compare
Pf6N2E2_2217 -1.1 -3.4 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_1396 -1.1 -2.1 ABC transporter, ATP-binding protein compare
Pf6N2E2_5226 -1.1 -1.3 FIG00955589: hypothetical protein compare
Pf6N2E2_3826 -1.1 -3.0 Nitrogen regulation protein NR(I) compare
Pf6N2E2_65 -1.1 -3.0 SAM-dependent methyltransferase YafE (UbiE paralog) compare
Pf6N2E2_3840 -1.1 -2.6 FIG00956267: hypothetical protein compare
Pf6N2E2_2072 -1.1 -3.2 hypothetical protein compare
Pf6N2E2_3004 -1.1 -1.3 Prophage baseplate assembly protein V compare
Pf6N2E2_1676 -1.1 -1.6 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_2261 -1.1 -4.4 VacJ-like lipoprotein precursor compare
Pf6N2E2_3260 -1.1 -3.6 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_5500 -1.1 -2.6 MoxR-like ATPases compare
Pf6N2E2_34 -1.1 -1.9 Cytosine deaminase (EC 3.5.4.1) compare
Pf6N2E2_4399 -1.1 -4.3 GGDEF domain/EAL domain protein compare
Pf6N2E2_5794 -1.1 -1.7 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_3534 -1.0 -1.3 Cobalamin biosynthesis protein CobG compare
Pf6N2E2_2794 -1.0 -1.6 putative membrane protein compare
Pf6N2E2_4751 -1.0 -2.5 response regulator compare
Pf6N2E2_4824 -1.0 -2.7 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_5261 -1.0 -0.9 FIG00956983: hypothetical protein compare
Pf6N2E2_70 -1.0 -2.8 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_193 -1.0 -2.8 hypothetical protein compare
Pf6N2E2_1450 -1.0 -1.9 FIG00960438: hypothetical protein compare
Pf6N2E2_2013 -1.0 -1.0 hypothetical protein compare
Pf6N2E2_3557 -1.0 -1.4 Chaperone-modulator protein CbpM compare
Pf6N2E2_1081 -1.0 -1.4 probable membrane protein YPO3302 compare
Pf6N2E2_6164 -1.0 -0.9 tRNA-Val-TAC compare
Pf6N2E2_2065 -1.0 -1.9 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_5190 -0.9 -4.7 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_2223 -0.9 -2.0 hypothetical protein compare
Pf6N2E2_664 -0.9 -1.5 Acetoin catabolism protein X compare
Pf6N2E2_1652 -0.9 -2.0 Transcriptional regulator, TetR family compare
Pf6N2E2_5178 -0.9 -1.5 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_75 -0.9 -2.7 Colicin V production protein compare
Pf6N2E2_3039 -0.9 -3.5 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_3537 -0.9 -2.7 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf6N2E2_494 -0.9 -1.4 Transcriptional regulator RutR of pyrimidine catabolism (TetR family) compare
Pf6N2E2_2795 -0.9 -2.0 putative exported protein compare
Pf6N2E2_4541 -0.9 -1.0 hypothetical protein compare
Pf6N2E2_2071 -0.9 -1.4 hypothetical protein compare
Pf6N2E2_2912 -0.9 -0.9 FIG00954094: hypothetical protein compare
Pf6N2E2_1784 -0.9 -2.2 Methyl-accepting chemotaxis protein compare
Pf6N2E2_1705 -0.9 -1.2 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_4083 -0.9 -1.7 hypothetical protein compare
Pf6N2E2_5219 -0.9 -4.9 FIG140336: TPR domain protein compare
Pf6N2E2_4488 -0.9 -3.4 Ribonucleotide reductase, alpha subunit compare
Pf6N2E2_3770 -0.9 -1.7 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_3204 -0.9 -1.6 Rho-specific inhibitor of transcription termination (YaeO) compare
Pf6N2E2_1022 -0.9 -1.3 Butyryl-CoA dehydrogenase (EC 1.3.99.2) compare
Pf6N2E2_4599 -0.9 -2.1 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_3589 -0.9 -1.8 Lysine decarboxylase family compare
Pf6N2E2_1671 -0.9 -2.7 Transcriptional regulator, TetR family compare
Pf6N2E2_4987 -0.8 -0.9 MaoC-like domain protein compare
Pf6N2E2_204 -0.8 -0.7 hypothetical protein compare
Pf6N2E2_3539 -0.8 -2.0 FIG00955840: hypothetical protein compare
Pf6N2E2_1095 -0.8 -1.5 Opine oxidase subunit C compare
Pf6N2E2_4025 -0.8 -1.2 hypothetical protein compare
Pf6N2E2_4372 -0.8 -3.0 FIG00460773: hypothetical protein compare
Pf6N2E2_953 -0.8 -2.2 FIG00958851: hypothetical protein compare
Pf6N2E2_2518 -0.8 -0.9 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_5772 -0.8 -2.0 DUF1232 domain-containing protein compare
Pf6N2E2_2513 -0.8 -2.7 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5546 -0.8 -3.5 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_1236 -0.8 -1.2 Transcriptional regulator, LysR family compare
Pf6N2E2_3325 -0.8 -1.6 ABC transporter, ATP-binding protein compare
Pf6N2E2_5510 -0.8 -1.6 FIG00955324: hypothetical protein compare
Pf6N2E2_2534 -0.8 -1.8 Cell division protein BolA compare
Pf6N2E2_1549 -0.8 -2.9 Transcriptional regulator, LysR family compare
Pf6N2E2_2852 -0.8 -3.7 GGDEF domain/EAL domain protein compare
Pf6N2E2_3014 -0.8 -1.5 P2-like prophage tail protein X compare
Pf6N2E2_3285 -0.8 -2.8 Rod shape-determining protein MreD compare
Pf6N2E2_3607 -0.8 -2.8 3'-to-5' exoribonuclease RNase R compare
Pf6N2E2_1761 -0.8 -1.7 Transcriptional regulator, LysR family compare
Pf6N2E2_1385 -0.8 -1.2 FIG00956261: hypothetical protein compare
Pf6N2E2_2586 -0.8 -1.3 Hpt domain protein compare
Pf6N2E2_1392 -0.8 -1.8 sensor histidine kinase compare
Pf6N2E2_3835 -0.8 -1.9 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_2756 -0.8 -3.0 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_613 -0.8 -1.0 lipoprotein, putative compare
Pf6N2E2_3198 -0.8 -0.9 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_2870 -0.8 -1.8 Ribonuclease BN (EC 3.1.-.-) compare
Pf6N2E2_1354 -0.8 -1.9 FIG026291: Hypothetical periplasmic protein compare
Pf6N2E2_1611 -0.8 -2.9 Phosphonate ABC transporter permease protein phnE2 (TC 3.A.1.9.1) compare
Pf6N2E2_5843 -0.8 -0.8 hypothetical protein compare
Pf6N2E2_490 -0.8 -1.7 PhaK-like protein compare
Pf6N2E2_114 -0.8 -1.8 Polysaccharide biosynthesis protein compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E2 in nitrogen source experiments

For nitrogen source L-Glutamic acid monopotassium salt monohydrate across organisms