Experiment set8IT005 for Marinobacter adhaerens HP15

marine broth with methylglyoxal 0.008 vol%

Group: stress
Media: marine_broth_2216 + methylglyoxal (0.008 vol%)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 933 B3,B4

Specific Phenotypes

For 9 genes in this experiment

For stress methylglyoxal in Marinobacter adhaerens HP15

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	2
Glutathione: Non-redox reactions	2
Methylglyoxal Metabolism	2
DNA-replication	1
DNA repair, bacterial	1
DNA repair, bacterial DinG and relatives	1
Glutathione-dependent pathway of formaldehyde detoxification	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation I	3	2	2
methylglyoxal degradation VIII	3	2	2
formaldehyde oxidation II (glutathione-dependent)	3	3	1
superpathway of methylglyoxal degradation	8	4	2
superpathway of C1 compounds oxidation to CO2	12	4	1