Experiment set8H38 for Pseudomonas stutzeri RCH2
RCH2 defined media with Carbenicillin disodium salt 0.02 mg/ml
Group: stressMedia: RCH2_defined_no_vitamin + Carbenicillin disodium salt (0.02 mg/ml), pH=7.2
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 9/23/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Growth plate: extra_min6 B7,B8
Specific Phenotypes
For 5 genes in this experiment
For stress Carbenicillin disodium salt in Pseudomonas stutzeri RCH2
For stress Carbenicillin disodium salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA Repair Base Excision | 1 |
| Pyruvate Alanine Serine Interconversions | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 2 | 1 |
| oxalate degradation II | 3 | 1 | 1 |
| NAD salvage pathway II (PNC IV cycle) | 5 | 4 | 1 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 4 | 1 |
| NAD salvage (plants) | 11 | 4 | 1 |