Experiment set8H36 for Pseudomonas stutzeri RCH2

Compare to:

RCH2 defined media with Benzethonium chloride 0.008 mM

Group: stress
Media: RCH2_defined_no_vitamin + Benzethonium chloride (0.008 mM), pH=7.2
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Adam on 9/23/2013
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM Sodium D,L-Lactate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Growth plate: extra_min6 A3,A4

Specific Phenotypes

For 35 genes in this experiment

For stress Benzethonium chloride in Pseudomonas stutzeri RCH2

For stress Benzethonium chloride across organisms

SEED Subsystems

Subsystem #Specific
Cobalt-zinc-cadmium resistance 2
Oxidative stress 2
Bacterial Chemotaxis 1
Bacterial hemoglobins 1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Flagellar motility 1
Folate Biosynthesis 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Lactate utilization 1
Lipid A modifications 1
Transcription factors bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
4-aminobenzoate biosynthesis I 2 2 1
L-glutamate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
cardiolipin biosynthesis II 3 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 4 1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas) 4 3 1
L-glutamate and L-glutamine biosynthesis 7 5 1
L-citrulline biosynthesis 8 7 1
chloramphenicol biosynthesis 9 1 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
superpathway of candicidin biosynthesis 11 4 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-citrulline metabolism 12 9 1
superpathway of cardiolipin biosynthesis (bacteria) 13 9 1
superpathway of chorismate metabolism 59 44 1