0.1X LB plate, Mixed culture; Enterobacter sp. TBS-079 (Tn7_Kan_Lux) 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct
Quality Metrics:
Time0 | 3/9/24 | which Time0s the sample was compared to |
cor12 | 0.32 | rank correlation(fit1, fit2), where fit1 is fitness for the first half (10-50%) and fit2 is fitness for the second half (50-90%) of each gene |
maxFit | 4.50 | The maximum fitness value |
opcor | 0.33 | rank correlation(upstream gene, downstream gene) over pairs that are adjacent and likely to be in the same operon |
adjcor | 0.08 | like opcor but for adjacent genes that are not on the same strand |
gccor | 0.06 | linear correlation of gene fitness and gene GC content |
mad12 | 0.33 | median absolute difference of fit1, fit2 |
mad12c | 1.07 | median absolute difference of log count for 1st and 2nd half of genes in this sample |
mad12c_t0 | 0.83 | like mad12c but for the Time0s |
gMed | 335 | median reads per gene in this sample |
gMedt0 | 734 | median reads per gene in the Time0 sample |
gMean | 1081 | mean reads per gene in this sample |
nMapped | 6.855 M | #reads for this sample that corresponded to a known strain (in millions) |
nPastEnd | 0.003 M | #reads that corresponded to a strain that has an insertion within the suicide vector instead of within the genome. |
nGenic | 4.615 M | #reads that lie within central 10-90% of a gene |
nUsed | 4.570 M | #reads used to estimate gene fitness (genic and enough coverage for strain and for gene) |
Specific Phenotypes
For 8 genes in this experiment
For mixed community Mixed culture; Enterobacter sp. TBS-079 (Tn7_Kan_Lux) 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct in Enterobacter sp. TBS_079
For mixed community Mixed culture; Enterobacter sp. TBS-079 (Tn7_Kan_Lux) 1:1 starting OD to Enterobacter_TBS_079_ML3; Collection-direct across organisms