Experiment set7IT089 for Phaeobacter inhibens DSM 17395
marine broth with Gentamicin sulfate salt 0.004 mg/ml
Group: stressMedia: marine_broth_2216 + Gentamicin sulfate salt (0.004 mg/ml)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on marchapr14
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 900 B5,B6
Specific Phenotypes
For 9 genes in this experiment
For stress Gentamicin sulfate salt in Phaeobacter inhibens DSM 17395
For stress Gentamicin sulfate salt across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Ammonia assimilation | 1 |
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
| Phosphate metabolism | 1 |
| Widespread colonization island | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate biosynthesis I | 2 | 2 | 2 |
| L-glutamine degradation I | 1 | 1 | 1 |
| L-glutamine degradation II | 1 | 1 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 2 |
| L-glutamate and L-glutamine biosynthesis | 7 | 7 | 2 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 4 | 1 |
| L-citrulline biosynthesis | 8 | 8 | 1 |
| superpathway of L-citrulline metabolism | 12 | 10 | 1 |