Experiment set7IT077 for Phaeobacter inhibens DSM 17395
marine broth with Dimethyl Sulfoxide 7.5 vol%
Group: stressMedia: marine_broth_2216 + Dimethyl Sulfoxide (7.5 vol%)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on marchapr14
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 898 B5,B6
Specific Phenotypes
For 4 genes in this experiment
For stress Dimethyl Sulfoxide in Phaeobacter inhibens DSM 17395
For stress Dimethyl Sulfoxide across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamine biosynthesis I | 1 | 1 | 1 |
| ammonia assimilation cycle II | 2 | 2 | 1 |
| ammonia assimilation cycle I | 2 | 2 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| superpathway of ammonia assimilation (plants) | 3 | 3 | 1 |
| L-aspartate degradation II (aerobic) | 3 | 1 | 1 |
| L-aspartate degradation III (anaerobic) | 3 | 1 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 7 | 1 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 10 | 1 |