Experiment set7IT075 for Marinobacter adhaerens HP15

marine broth with Zinc sulfate heptahydrate 0.5 mM

Group: stress
Media: marine_broth_2216 + Zinc sulfate heptahydrate (0.5 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 930 A5,A6

Specific Phenotypes

For 17 genes in this experiment

For stress Zinc sulfate heptahydrate in Marinobacter adhaerens HP15

For stress Zinc sulfate heptahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cobalt-zinc-cadmium resistance	2
Arginine and Ornithine Degradation	1
Biogenesis of c-type cytochromes	1
Flagellar motility	1
Flagellum	1
Glutathione: Non-redox reactions	1
Lipid A modifications	1
Methylglyoxal Metabolism	1
Periplasmic disulfide interchange	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
RNA processing and degradation, bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation VIII	3	2	1
methylglyoxal degradation I	3	2	1
tRNA processing	10	10	2
superpathway of methylglyoxal degradation	8	4	1