Experiment set7IT074 for Phaeobacter inhibens DSM 17395

marine broth with Phosphomycin disodium salt 1 mg/ml

Group: stress
Media: marine_broth_2216 + Phosphomycin disodium salt (1 mg/ml)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on marchapr14
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 898 A1,A2

Specific Phenotypes

For 25 genes in this experiment

For stress Phosphomycin disodium salt in Phaeobacter inhibens DSM 17395

For stress Phosphomycin disodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Alkylphosphonate utilization	8
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	4
High affinity phosphate transporter and control of PHO regulon	4
Phosphate metabolism	4
2-phosphoglycolate salvage	1
Glycolate, glyoxylate interconversions	1
Methionine Biosynthesis	1
Photorespiration (oxidative C2 cycle)	1
YjeE	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Methionine metabolism
• Cysteine metabolism
• Pentose phosphate pathway
• Purine metabolism
• Selenoamino acid metabolism
• Glyoxylate and dicarboxylate metabolism
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-homocysteine biosynthesis	2	2	1
superpathway of L-cysteine biosynthesis (fungi)	6	4	3
homocysteine and cysteine interconversion	4	2	2
L-cysteine biosynthesis III (from L-homocysteine)	2	1	1
methylphosphonate degradation I	5	2	2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	8	3
glyphosate degradation III	7	4	2
guanosine ribonucleotides de novo biosynthesis	4	4	1
L-methionine biosynthesis III	4	4	1
(aminomethyl)phosphonate degradation	8	4	2
arsenic detoxification (bacteria)	4	2	1
L-methionine biosynthesis I	5	4	1
dZTP biosynthesis	5	3	1
superpathway of L-cysteine biosynthesis (mammalian)	5	3	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III	5	3	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II	5	2	1
methylphosphonate degradation II	5	1	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
L-methionine biosynthesis II	6	4	1
arsenate detoxification I	6	3	1
L-cysteine biosynthesis VI (reverse transsulfuration)	7	3	1
superpathway of L-homoserine and L-methionine biosynthesis	8	7	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	7	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	8	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	8	1
photorespiration I	9	7	1
photorespiration III	9	7	1
photorespiration II	10	7	1
peptidoglycan recycling II	10	2	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	12	1
superpathway of purine nucleotide salvage	14	13	1
peptidoglycan recycling I	14	8	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	12	1
superpathway of L-methionine salvage and degradation	16	9	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
aspartate superpathway	25	23	1
superpathway of purine nucleotides de novo biosynthesis II	26	23	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	42	1