Experiment set7IT063 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant medium with Tetrazolium violet 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Tetrazolium violet (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 5/2/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2032 D1

Specific Phenotypes

None in this experiment

For Bacteroides thetaiotaomicron VPI-5482 in stress experiments

For stress Tetrazolium violet across organisms