Experiment set7IT061 for Marinobacter adhaerens HP15
marine broth with Bacitracin 0.5 mg/ml
Group: stressMedia: marine_broth_2216 + Bacitracin (0.5 mg/ml)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 929 E7,E8
Specific Phenotypes
For 5 genes in this experiment
For stress Bacitracin in Marinobacter adhaerens HP15
For stress Bacitracin across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Anaerobic respiratory reductases | 1 |
| Arsenic resistance | 1 |
| Lipid A-Ara4N pathway ( Polymyxin resistance ) | 1 |
| Teichuronic acid biosynthesis | 1 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose and glucuronate interconversions
- Ascorbate and aldarate metabolism
- Purine metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| UDP-α-D-glucuronate biosynthesis (from UDP-glucose) | 1 | 1 | 1 |
| UDP-α-D-xylose biosynthesis | 2 | 2 | 1 |
| arsenate detoxification III | 2 | 1 | 1 |
| arsenic detoxification (bacteria) | 4 | 2 | 1 |
| superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis | 6 | 4 | 1 |
| arsenate detoxification I | 6 | 3 | 1 |
| arsenic detoxification (plants) | 6 | 2 | 1 |
| UDP-sugars interconversion | 9 | 3 | 1 |
| teichuronic acid biosynthesis (B. subtilis 168) | 9 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 8 | 1 |
| arsenic detoxification (yeast) | 12 | 3 | 1 |