Experiment set7IT060 for Klebsiella michiganensis M5al

LB with Sodium perchlorate monohydrate 100 mM

Group: stress
Media: LB + Sodium perchlorate monohydrate (100 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1803 C5

Specific Phenotypes

For 21 genes in this experiment

For stress Sodium perchlorate monohydrate in Klebsiella michiganensis M5al

For stress Sodium perchlorate monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	6
Molybdenum cofactor biosynthesis	3
Peptidoglycan Biosynthesis	2
CytR regulation	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Transport of Molybdenum	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Peptidoglycan biosynthesis
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Lysine degradation
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Keratan sulfate biosynthesis
• Glycerolipid metabolism
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series
• Pyruvate metabolism
• Biotin metabolism
• Carotenoid biosynthesis - General
• Zeatin biosynthesis
• Nitrogen metabolism
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Flavone and flavonol biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bis(guanylyl molybdenum cofactor) biosynthesis	2	2	2
guanylyl molybdenum cofactor biosynthesis	1	1	1
L-malate degradation II	1	1	1
bis(guanylyl tungstenpterin) cofactor biosynthesis	1	1	1
nitrate reduction VIIIb (dissimilatory)	2	2	1
nitrate reduction VIII (dissimilatory)	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
NADH to nitrate electron transfer	2	2	1
nitrate reduction IX (dissimilatory)	2	2	1
malate/L-aspartate shuttle pathway	2	2	1
molybdenum cofactor biosynthesis	3	3	1
L-carnitine degradation II	3	1	1
chitin deacetylation	4	3	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	5	3
bis(tungstenpterin) cofactor biosynthesis	4	1	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	8	2
glyoxylate cycle	6	6	1
TCA cycle VIII (Chlamydia)	6	5	1
molybdopterin biosynthesis	6	5	1
methylgallate degradation	6	4	1
gluconeogenesis I	13	13	2
incomplete reductive TCA cycle	7	5	1
anaerobic energy metabolism (invertebrates, cytosol)	7	5	1
pyruvate fermentation to propanoate I	7	5	1
protocatechuate degradation I (meta-cleavage pathway)	8	6	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
superpathway of anaerobic energy metabolism (invertebrates)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
TCA cycle II (plants and fungi)	9	7	1
TCA cycle IV (2-oxoglutarate decarboxylase)	9	7	1
TCA cycle V (2-oxoglutarate synthase)	9	7	1
TCA cycle I (prokaryotic)	10	9	1
TCA cycle III (animals)	10	8	1
peptidoglycan recycling II	10	7	1
superpathway of vanillin and vanillate degradation	10	7	1
anaerobic energy metabolism (invertebrates, mitochondrial)	10	7	1
reductive TCA cycle I	11	8	1
L-glutamate degradation VIII (to propanoate)	11	7	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
superpathway of glyoxylate bypass and TCA	12	11	1
gluconeogenesis III	12	9	1
reductive TCA cycle II	12	7	1
syringate degradation	12	6	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	10	1
formaldehyde assimilation I (serine pathway)	13	8	1
peptidoglycan recycling I	14	14	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1
mixed acid fermentation	16	16	1
methylaspartate cycle	19	12	1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	18	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	25	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	23	1