Experiment set7IT058 for Marinobacter adhaerens HP15

marine broth with Aluminum chloride hydrate 1 mM

Group: stress
Media: marine_broth_2216 + Aluminum chloride hydrate (1 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 929 D3,D4

Specific Phenotypes

For 28 genes in this experiment

For stress Aluminum chloride hydrate in Marinobacter adhaerens HP15

For stress Aluminum chloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Lipid A-Ara4N pathway ( Polymyxin resistance )	3
Biogenesis of c-type cytochromes	1
DNA repair, bacterial	1
Glutathione: Non-redox reactions	1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway	1
Lactose and Galactose Uptake and Utilization	1
Lipid A modifications	1
Methylglyoxal Metabolism	1
N-linked Glycosylation in Bacteria	1
Orphan regulatory proteins	1
Periplasmic disulfide interchange	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Rhamnose containing glycans	1
Teichoic and lipoteichoic acids biosynthesis	1
Teichuronic acid biosynthesis	1
Ubiquinone Biosynthesis	1
linker unit-arabinogalactan synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Histidine metabolism
• Phenylalanine metabolism
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Tryptophan metabolism
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• Lipopolysaccharide biosynthesis
• Pyruvate metabolism
• Toluene and xylene degradation
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• 1,4-Dichlorobenzene degradation
• Benzoate degradation via CoA ligation
• Styrene degradation
• Methane metabolism
• Limonene and pinene degradation
• Brassinosteroid biosynthesis
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Isoflavonoid biosynthesis
• Alkaloid biosynthesis I
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-D-galactosamine biosynthesis I	1	1	1
UDP-α-D-glucuronate biosynthesis (from UDP-glucose)	1	1	1
UDP-α-D-galactose biosynthesis	1	1	1
UDP-α-D-xylose biosynthesis	2	2	1
UDP-α-D-galactofuranose biosynthesis	2	1	1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis	6	4	2
methylglyoxal degradation VIII	3	2	1
methylglyoxal degradation I	3	2	1
D-galactose detoxification	3	1	1
UDP-sugars interconversion	9	3	2
teichuronic acid biosynthesis (B. subtilis 168)	9	2	2
D-galactose degradation I (Leloir pathway)	5	2	1
colanic acid building blocks biosynthesis	11	8	2
UDP-N-acetyl-D-galactosamine biosynthesis II	7	4	1
stachyose degradation	7	2	1
lipoprotein posttranslational modification (Gram-negative bacteria)	7	2	1
superpathway of methylglyoxal degradation	8	4	1
tunicamycin biosynthesis	9	3	1
poly(glycerol phosphate) wall teichoic acid biosynthesis	11	2	1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	18	1	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	12	1