Experiment set7IT057 for Klebsiella michiganensis M5al

LB with Sisomicin sulfate salt 0.008 mg/ml

Group: stress
Media: LB + Sisomicin sulfate salt (0.008 mg/ml)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1803 B2

Specific Phenotypes

For 58 genes in this experiment

For stress Sisomicin sulfate salt in Klebsiella michiganensis M5al

For stress Sisomicin sulfate salt across organisms

SEED Subsystems

Subsystem	#Specific
Conserved gene cluster associated with Met-tRNA formyltransferase	2
Heat shock dnaK gene cluster extended	2
Molybdenum cofactor biosynthesis	2
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	2
Polyamine Metabolism	2
tRNA processing	2
Bacterial Cytoskeleton	1
Flavodoxin	1
Formate hydrogenase	1
Glutathionylspermidine and Trypanothione	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Methionine Biosynthesis	1
Methionine Degradation	1
Protein degradation	1
Purine nucleotide synthesis regulator	1
Pyridoxin (Vitamin B6) Biosynthesis	1
Staphylococcal pathogenicity islands SaPI	1
Sucrose utilization	1
Thioredoxin-disulfide reductase	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Puromycin biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Ubiquinone and menaquinone biosynthesis
• Pyrimidine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Aminophosphonate metabolism
• High-mannose type N-glycan biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• alpha-Linolenic acid metabolism
• Sphingolipid metabolism
• Naphthalene and anthracene degradation
• Glyoxylate and dicarboxylate metabolism
• Trinitrotoluene degradation
• Methane metabolism
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
bis(guanylyl molybdenum cofactor) biosynthesis	2	2	2
guanylyl molybdenum cofactor biosynthesis	1	1	1
bis(guanylyl tungstenpterin) cofactor biosynthesis	1	1	1
formate oxidation to CO2	1	1	1
glutathionylspermidine biosynthesis	1	1	1
formate to dimethyl sulfoxide electron transfer	2	2	1
nitrate reduction III (dissimilatory)	2	2	1
pseudouridine degradation	2	2	1
formate to trimethylamine N-oxide electron transfer	2	1	1
cardiolipin biosynthesis I	3	3	1
cardiolipin biosynthesis II	3	3	1
molybdenum cofactor biosynthesis	3	3	1
formate to nitrite electron transfer	3	1	1
queuosine biosynthesis I (de novo)	4	4	1
cardiolipin and phosphatidylethanolamine biosynthesis (Xanthomonas)	4	3	1
bis(tungstenpterin) cofactor biosynthesis	4	1	1
oxalate degradation VI	4	1	1
enterobacterial common antigen biosynthesis	5	4	1
queuosine biosynthesis III (queuosine salvage)	5	3	1
oxalate degradation III	5	1	1
molybdopterin biosynthesis	6	5	1
superpathway of cardiolipin biosynthesis (bacteria)	13	10	2
tRNA processing	10	10	1
superpathway of enterobacterial common antigen biosynthesis	10	9	1
superpathway of phospholipid biosynthesis III (E. coli)	12	11	1
superpathway of C1 compounds oxidation to CO2	12	4	1
purine nucleobases degradation I (anaerobic)	15	6	1
purine nucleobases degradation II (anaerobic)	24	17	1