Experiment set7IT055 for Marinobacter adhaerens HP15

marine broth with Sisomicin sulfate salt 0.008 mg/ml

Group: stress
Media: marine_broth_2216 + Sisomicin sulfate salt (0.008 mg/ml)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jake on 5/20/2014
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 929 B1,B2

Specific Phenotypes

For 10 genes in this experiment

For stress Sisomicin sulfate salt in Marinobacter adhaerens HP15

For stress Sisomicin sulfate salt across organisms

SEED Subsystems

Subsystem	#Specific
Heat shock dnaK gene cluster extended	2
Glycine reductase, sarcosine reductase and betaine reductase	1
Phosphate metabolism	1
Queuosine-Archaeosine Biosynthesis	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
queuosine biosynthesis I (de novo)	4	4	1
preQ0 biosynthesis	4	2	1
tRNA processing	10	10	2
adipate degradation	5	5	1
fatty acid salvage	6	6	1
oleate β-oxidation	35	30	3
2-methyl-branched fatty acid β-oxidation	14	10	1