marine broth with Doxycycline hyclate 0.002 mg/ml
Group:
stress
Media:
marine_broth_2216 +
Doxycycline hyclate (0.002 mg/ml)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Adam on
marchapr14
Media components: 5 g/L Bacto Peptone, 1 g/L
Yeast Extract, 0.1 g/L
Ferric citrate, 19.45 g/L
Sodium Chloride, 5.9 g/L
Magnesium chloride hexahydrate, 3.24 g/L
Magnesium sulfate, 1.8 g/L
Calcium chloride, 0.55 g/L
Potassium Chloride, 0.16 g/L
Sodium bicarbonate, 0.08 g/L
Potassium bromide, 34 mg/L
Strontium chloride, 22 mg/L
Boric Acid, 4 mg/L
Sodium metasilicate, 2.4 mg/L
sodium fluoride, 8 mg/L
Disodium phosphate
Growth plate: 897 F3,F4
Specific Phenotypes
For 5 genes in this experiment
For stress Doxycycline hyclate in Phaeobacter inhibens DSM 17395
For stress Doxycycline hyclate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 6 | 2 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 10 | 3 |
| adipate degradation | 5 | 5 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 3 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 1 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| superpathway of phenylethylamine degradation | 11 | 6 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 5 | 2 |
| oleate β-oxidation | 35 | 30 | 6 |
| fatty acid salvage | 6 | 6 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 3 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| L-valine degradation I | 8 | 5 | 1 |
| pyruvate fermentation to butanol I | 8 | 3 | 1 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 4 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 3 | 2 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 10 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 5 | 1 |
| glycerol degradation to butanol | 16 | 9 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 11 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |