Experiment set7IT051 for Sphingomonas koreensis DSMZ 15582

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N-Acetyl-D-Glucosamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + N-Acetyl-D-Glucosamine (5 mM)
Culturing: korea_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Valentine on 10/5/22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 7 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Sphingomonas koreensis DSMZ 15582

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 3
Sialic Acid Metabolism 2
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 2
Cobalt-zinc-cadmium resistance 1
Fructose and Mannose Inducible PTS 1
Fructose utilization 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Mannitol Utilization 1
Photorespiration (oxidative C2 cycle) 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 2
N-acetylglucosamine degradation II 3 2 2
glycerophosphodiester degradation 2 2 1
glycerol-3-phosphate shuttle 2 2 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 2 1
glycerol-3-phosphate to fumarate electron transfer 2 1 1
glycerol-3-phosphate to hydrogen peroxide electron transport 2 1 1
glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer 2 1 1
nitrate reduction IX (dissimilatory) 2 1 1
glycine biosynthesis II 3 3 1
glycine cleavage 3 3 1
glycerol degradation I 3 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 2 2
sn-glycerol 3-phosphate anaerobic respiration 3 1 1
glycerol and glycerophosphodiester degradation 4 3 1
chitin derivatives degradation 8 2 2
D-galactosamine and N-acetyl-D-galactosamine degradation 4 1 1
UDP-N-acetyl-D-glucosamine biosynthesis I 5 5 1
N-acetyl-D-galactosamine degradation 5 1 1
UDP-N-acetyl-D-glucosamine biosynthesis II 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis III 6 2 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
chitin degradation I (archaea) 7 1 1
chitin biosynthesis 9 6 1
CMP-legionaminate biosynthesis I 10 2 1
superpathway of N-acetylneuraminate degradation 22 14 2
O-antigen building blocks biosynthesis (E. coli) 11 7 1
peptidoglycan recycling I 14 9 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 9 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 9 1