Experiment set7IT048 for Klebsiella michiganensis M5al

LB with Paraquat dichloride 0.1 mg/ml

Group: stress
Media: LB + Paraquat dichloride (0.1 mg/ml)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1801 C2

Specific Phenotypes

For 22 genes in this experiment

For stress Paraquat dichloride in Klebsiella michiganensis M5al

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	3
Selenocysteine metabolism	2
Thioredoxin-disulfide reductase	2
ATP-dependent RNA helicases, bacterial	1
Heat shock dnaK gene cluster extended	1
Iron acquisition in Vibrio	1
Nitrosative stress	1
Oxidative stress	1
Queuosine-Archaeosine Biosynthesis	1
RNA processing and degradation, bacterial	1
Rrf2 family transcriptional regulators	1
Siderophore Enterobactin	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Selenoamino acid metabolism
• Methionine metabolism
• Glutathione metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Aminoacyl-tRNA biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-selenocysteine biosynthesis I (bacteria)	3	3	1
molybdenum cofactor biosynthesis	3	3	1
tRNA processing	10	10	2