Experiment set7IT042 for Klebsiella michiganensis M5al

LB with Cobalt chloride hexahydrate 0.5 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.5 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1801 A5

Specific Phenotypes

For 17 genes in this experiment

For stress Cobalt chloride hexahydrate in Klebsiella michiganensis M5al

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	3
Copper homeostasis: copper tolerance	2
Phosphate metabolism	2
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Biogenesis of c-type cytochromes	1
Campylobacter Iron Metabolism	1
Chorismate Synthesis	1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)	1
Polyamine Metabolism	1
Ribonucleotide reduction	1
Synechocystis experimental	1
Triacylglycerol metabolism	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups
• Purine metabolism
• Arginine and proline metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Glutathione metabolism
• Glycerophospholipid metabolism
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
guanosine deoxyribonucleotides de novo biosynthesis II	4	4	2
adenosine deoxyribonucleotides de novo biosynthesis II	4	4	2
putrescine biosynthesis I	2	2	1
pseudouridine degradation	2	2	1
3-dehydroquinate biosynthesis I	2	2	1
adenosine deoxyribonucleotides de novo biosynthesis I	2	2	1
guanosine deoxyribonucleotides de novo biosynthesis I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
molybdenum cofactor biosynthesis	3	3	1
putrescine biosynthesis II	3	1	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	1	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	4
superpathway of adenosine nucleotides de novo biosynthesis II	7	7	2
superpathway of guanosine nucleotides de novo biosynthesis II	8	8	2
superpathway of putrescine biosynthesis	4	3	1
spermidine biosynthesis III	4	2	1
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
pyrimidine deoxyribonucleotides de novo biosynthesis I	9	9	2
pyrimidine deoxyribonucleotides de novo biosynthesis III	9	8	2
superpathway of adenosine nucleotides de novo biosynthesis I	5	5	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
superpathway of purine nucleotides de novo biosynthesis II	26	26	4
chorismate biosynthesis I	7	7	1
pyrimidine deoxyribonucleotides de novo biosynthesis IV	7	6	1
superpathway of polyamine biosynthesis I	8	7	1
pyrimidine deoxyribonucleotides biosynthesis from CTP	8	6	1
superpathway of polyamine biosynthesis II	8	4	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	2
superpathway of L-tyrosine biosynthesis	10	10	1
superpathway of L-phenylalanine biosynthesis	10	10	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	2
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	11	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	4
superpathway of L-tryptophan biosynthesis	13	13	1
superpathway of L-arginine and L-ornithine degradation	13	13	1
superpathway of purine nucleotide salvage	14	13	1
superpathway of arginine and polyamine biosynthesis	17	16	1
superpathway of aromatic amino acid biosynthesis	18	18	1
superpathway of chorismate metabolism	59	54	1