Experiment set7IT041 for Paraburkholderia bryophila 376MFSha3.1

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Azelaic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Azelaic acid (10 mM)
Culturing: Burk376_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 1.3 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For carbon source Azelaic acid in Paraburkholderia bryophila 376MFSha3.1

For carbon source Azelaic acid across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 2
Flagellar motility 1
Flagellum 1
Menaquinone and Phylloquinone Biosynthesis 1
Transcription initiation, bacterial sigma factors 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
2-carboxy-1,4-naphthoquinol biosynthesis 7 1 1
benzoyl-CoA degradation III (anaerobic) 9 3 1
superpathway of demethylmenaquinol-8 biosynthesis I 9 2 1
superpathway of demethylmenaquinol-6 biosynthesis I 9 1 1
superpathway of demethylmenaquinol-9 biosynthesis 9 1 1
superpathway of menaquinol-8 biosynthesis I 10 3 1
superpathway of menaquinol-6 biosynthesis 10 2 1
superpathway of menaquinol-11 biosynthesis 10 2 1
superpathway of menaquinol-10 biosynthesis 10 2 1
superpathway of menaquinol-13 biosynthesis 10 2 1
superpathway of menaquinol-7 biosynthesis 10 2 1
superpathway of menaquinol-12 biosynthesis 10 2 1
superpathway of menaquinol-9 biosynthesis 10 2 1
superpathway of phylloquinol biosynthesis 15 2 1
superpathway of chorismate metabolism 59 43 1