Experiment set7IT037 for Pseudomonas fluorescens FW300-N2E2
LB with Calcium chloride dihydrate 0.0125 M
Group: stressMedia: LB + Calcium chloride dihydrate (0.0125 M)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/5/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Specific Phenotypes
For 6 genes in this experiment
For stress Calcium chloride dihydrate in Pseudomonas fluorescens FW300-N2E2
For stress Calcium chloride dihydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| DNA repair, UvrABC system | 2 |
| Peptidoglycan Biosynthesis | 1 |
| Type IV pilus | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 2 |
| peptidoglycan biosynthesis V (β-lactam resistance) | 17 | 11 | 2 |
| peptidoglycan biosynthesis I (meso-diaminopimelate containing) | 12 | 12 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 1 |
| peptidoglycan biosynthesis III (mycobacteria) | 15 | 11 | 1 |