Experiment set7IT028 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Sisomicin sulfate salt 0.0016 mg/ml

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_4987 +2.7 6.2 MaoC-like domain protein compare
Pf6N2E2_3941 +2.7 9.1 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3663 +2.6 17.8 Glycosyl transferase compare
Pf6N2E2_6057 +2.6 8.9 FIG002781: Alpha-L-glutamate ligase family protein compare
Pf6N2E2_2310 +2.5 15.8 Cytochrome c oxidase subunit CcoN (EC 1.9.3.1) compare
Pf6N2E2_2317 +2.4 14.6 Putative analog of CcoH, COG3198 compare
Pf6N2E2_2311 +2.4 15.2 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf6N2E2_3942 +2.4 17.9 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_3661 +2.4 14.6 hypothetical protein compare
Pf6N2E2_2318 +2.3 15.5 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_4464 +2.3 4.1 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_6058 +2.2 12.0 FIG139976: hypothetical protein compare
Pf6N2E2_4159 +2.2 8.3 Cytochrome c4 compare
Pf6N2E2_2320 +2.1 9.9 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_3659 +2.1 7.2 Protein fixF compare
Pf6N2E2_5339 +2.1 11.5 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_2313 +2.1 7.2 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_2447 +2.0 12.5 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_2527 +2.0 9.2 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_5527 +2.0 3.8 Cold shock protein CspC compare
Pf6N2E2_3786 +1.9 7.5 Twin-arginine translocation protein TatC compare
Pf6N2E2_2552 +1.9 11.5 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_2973 +1.9 10.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3664 +1.8 11.0 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2309 +1.8 5.0 hypothetical protein compare
Pf6N2E2_3271 +1.8 6.9 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3835 +1.8 7.8 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_5338 +1.8 9.7 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_5219 +1.7 10.3 FIG140336: TPR domain protein compare
Pf6N2E2_5943 +1.7 3.0 hypothetical protein compare
Pf6N2E2_1656 +1.7 2.3 hypothetical protein compare
Pf6N2E2_5337 +1.7 5.8 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_5986 +1.7 5.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2022 +1.6 9.1 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_5554 +1.6 4.7 FIG023103: Predicted transmembrane protein compare
Pf6N2E2_5318 +1.4 13.1 Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-) compare
Pf6N2E2_5987 +1.4 7.1 OmpA family protein compare
Pf6N2E2_5317 +1.3 9.8 Cytochrome O ubiquinol oxidase subunit II (EC 1.10.3.-) compare
Pf6N2E2_2428 +1.3 5.3 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_5705 +1.3 4.1 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_5320 +1.2 8.1 Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-) compare
Pf6N2E2_2506 +1.2 10.1 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_5321 +1.2 8.7 Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB compare
Pf6N2E2_3782 +1.2 3.1 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_4438 +1.2 4.4 hypothetical protein compare
Pf6N2E2_3658 +1.2 5.7 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_5319 +1.2 8.2 Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-) compare
Pf6N2E2_3328 +1.2 1.5 transcriptional regulator compare
Pf6N2E2_4071 +1.2 5.8 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4451 +1.2 6.1 FIG139438: lipoprotein B compare
Pf6N2E2_2316 +1.1 6.1 Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation compare
Pf6N2E2_2509 +1.1 2.9 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_5156 +1.1 3.7 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2534 +1.0 3.5 Cell division protein BolA compare
Pf6N2E2_3560 +1.0 7.2 membrane protein, putative compare
Pf6N2E2_5845 +1.0 5.4 Nucleoside ABC transporter, periplasmic nucleoside-binding protein compare
Pf6N2E2_961 +1.0 2.8 FIG00954712: hypothetical protein compare
Pf6N2E2_4305 +1.0 2.2 FIG00959590: hypothetical protein compare
Pf6N2E2_2312 +1.0 2.2 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_2674 +0.9 1.2 FIG00953856: hypothetical protein compare
Pf6N2E2_4932 +0.9 5.4 hypothetical protein compare
Pf6N2E2_3785 +0.9 2.6 Twin-arginine translocation protein TatB compare
Pf6N2E2_3303 +0.9 4.2 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf6N2E2_1606 +0.9 4.0 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_3634 +0.9 2.4 Transporter compare
Pf6N2E2_2189 +0.9 4.1 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_2586 +0.9 2.2 Hpt domain protein compare
Pf6N2E2_5846 +0.9 6.4 Nucleoside ABC transporter, periplasmic nucleoside-binding protein compare
Pf6N2E2_579 +0.9 1.8 FIG00962753: hypothetical protein compare
Pf6N2E2_2499 +0.9 2.5 Competence protein ComEA helix-hairpin-helix region precursor compare
Pf6N2E2_5720 +0.8 6.0 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_2156 +0.8 1.6 Putative polyketide synthase (Fragment) compare
Pf6N2E2_4943 +0.8 1.6 PspA/IM30 family protein compare
Pf6N2E2_170 +0.8 1.6 lipoprotein, putative compare
Pf6N2E2_3856 +0.8 4.0 Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1) compare
Pf6N2E2_3541 +0.8 2.9 Putative multicopper oxidases compare
Pf6N2E2_4554 +0.8 6.2 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_6087 +0.8 2.2 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_5153 +0.8 4.2 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_3872 +0.8 1.6 hypothetical protein compare
Pf6N2E2_2124 +0.8 1.8 Mg(2+) transport ATPase protein C compare
Pf6N2E2_4063 +0.8 2.5 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf6N2E2_3247 +0.8 4.1 Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52) compare
Pf6N2E2_5840 +0.8 2.7 hypothetical protein compare
Pf6N2E2_2440 +0.8 1.4 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf6N2E2_4107 +0.8 5.9 Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone compare
Pf6N2E2_3542 +0.7 5.8 Outer membrane receptor proteins, mostly Fe transport compare
Pf6N2E2_3033 +0.7 2.0 hypothetical protein compare
Pf6N2E2_5158 +0.7 2.6 FIG00954153: hypothetical protein compare
Pf6N2E2_1329 +0.7 2.6 Phenylacetaldehyde dehydrogenase (EC 1.2.1.39) compare
Pf6N2E2_4803 +0.7 1.4 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_2507 +0.7 3.8 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_2794 +0.7 1.4 putative membrane protein compare
Pf6N2E2_478 +0.7 1.3 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) compare
Pf6N2E2_1797 +0.7 1.9 Transcriptional regulator compare
Pf6N2E2_4452 +0.7 4.4 Porphobilinogen synthase (EC 4.2.1.24) compare
Pf6N2E2_1566 +0.7 1.9 FIG00960770: hypothetical protein compare
Pf6N2E2_2519 +0.7 1.2 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_2755 +0.7 1.3 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_2493 +0.7 3.9 Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1) compare
Pf6N2E2_773 +0.7 3.9 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_4540 +0.7 1.8 hypothetical protein compare
Pf6N2E2_1147 +0.7 1.7 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_3179 +0.7 1.0 FIG021952: putative membrane protein compare
Pf6N2E2_3060 +0.7 1.8 Outer membrane protein H precursor compare
Pf6N2E2_4570 +0.7 1.9 Cation/multidrug efflux pump compare
Pf6N2E2_924 +0.7 1.5 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_3539 +0.7 1.2 FIG00955840: hypothetical protein compare
Pf6N2E2_2193 +0.7 3.0 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (from data) compare
Pf6N2E2_55 +0.6 1.6 Probable hydrolase compare
Pf6N2E2_1759 +0.6 2.0 uncharacterized domain 1 compare
Pf6N2E2_1683 +0.6 1.1 Fe2+-dicitrate sensor, membrane component compare
Pf6N2E2_145 +0.6 4.0 putative exported protein compare
Pf6N2E2_241 +0.6 0.9 hypothetical protein compare
Pf6N2E2_2314 +0.6 1.6 VapB protein (antitoxin to VapC) compare
Pf6N2E2_1824 +0.6 1.4 hypothetical protein compare
Pf6N2E2_5583 +0.6 2.8 Bacterioferritin compare
Pf6N2E2_3346 +0.6 3.1 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_699 +0.6 1.7 Heme d1 biosynthesis protein NirG compare
Pf6N2E2_4263 +0.6 1.4 Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family compare
Pf6N2E2_3829 +0.6 2.3 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf6N2E2_5226 +0.6 1.3 FIG00955589: hypothetical protein compare
Pf6N2E2_3283 +0.6 3.1 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_2864 +0.6 1.1 Gluconokinase (EC 2.7.1.12) compare
Pf6N2E2_2999 +0.6 1.5 probable tail fiber assembly protein compare
Pf6N2E2_5157 +0.6 4.6 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_1506 +0.6 1.9 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_3443 +0.6 2.4 Sulfate permease compare
Pf6N2E2_2473 +0.6 0.9 Major outer membrane lipoprotein I compare
Pf6N2E2_2526 +0.6 1.6 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_1212 +0.6 2.5 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_1776 +0.6 1.4 Chemotaxis protein CheD compare
Pf6N2E2_953 +0.6 1.0 FIG00958851: hypothetical protein compare
Pf6N2E2_3947 +0.6 1.4 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_4630 +0.6 2.1 Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-) compare
Pf6N2E2_315 +0.6 2.3 probable transmembrane protein compare
Pf6N2E2_5936 +0.6 1.6 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_3088 +0.6 0.8 hypothetical protein compare
Pf6N2E2_2682 +0.6 1.6 Transcriptional regulator, LysR family compare
Pf6N2E2_6059 +0.6 2.7 FIG008443: hypothetical protein compare
Pf6N2E2_923 +0.6 1.8 Haloacid dehalogenase, type II (EC 3.8.1.2) compare
Pf6N2E2_5671 +0.6 2.3 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_5155 +0.6 2.1 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_5111 +0.6 2.0 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_748 +0.6 1.6 Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine compare
Pf6N2E2_3723 +0.6 2.5 FIG022199: FAD-binding protein compare
Pf6N2E2_2126 +0.6 2.9 4Fe-4S ferredoxin, iron-sulfur binding compare
Pf6N2E2_2448 +0.6 2.3 Thiopurine S-methyltransferase (EC 2.1.1.67) compare
Pf6N2E2_3351 +0.6 2.6 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_4467 +0.6 1.4 hypothetical protein compare
Pf6N2E2_1124 +0.6 1.5 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_2384 +0.5 1.5 CmpX compare
Pf6N2E2_2323 +0.5 1.3 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_1560 +0.5 1.4 Glycine cleavage system transcriptional activator GcvA compare
Pf6N2E2_987 +0.5 1.2 hypothetical protein compare
Pf6N2E2_5707 +0.5 3.7 Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate compare
Pf6N2E2_1605 +0.5 3.1 Carboxynorspermidine dehydrogenase, putative (EC 1.1.1.-) compare
Pf6N2E2_2675 +0.5 0.9 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_689 +0.5 1.3 FIG00956189: hypothetical protein compare
Pf6N2E2_1136 +0.5 0.8 Transcriptional regulator, TetR family compare
Pf6N2E2_1085 +0.5 1.7 Fe2+/Zn2+ uptake regulation proteins compare
Pf6N2E2_1339 +0.5 0.9 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_2633 +0.5 1.7 Nitroreductase family protein compare
Pf6N2E2_1104 +0.5 3.0 Gluconate dehydratase (EC 4.2.1.39) compare
Pf6N2E2_2324 +0.5 1.7 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf6N2E2_4453 +0.5 4.7 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_1371 +0.5 1.5 hypothetical protein compare
Pf6N2E2_4698 +0.5 3.2 putative DNA binding protein compare
Pf6N2E2_4025 +0.5 1.1 hypothetical protein compare
Pf6N2E2_1509 +0.5 2.6 FIG00450070: hypothetical protein compare
Pf6N2E2_4298 +0.5 2.5 Uncharacterized protein ImpB compare
Pf6N2E2_367 +0.5 1.3 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_1870 +0.5 1.6 hypothetical protein compare
Pf6N2E2_2018 +0.5 1.1 hypothetical protein compare
Pf6N2E2_1935 +0.5 1.3 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_1082 +0.5 1.6 CopG protein compare
Pf6N2E2_715 +0.5 1.9 putative membrane protein compare
Pf6N2E2_1199 +0.5 1.6 hypothetical protein compare
Pf6N2E2_4827 +0.5 2.4 Lipoate-protein ligase A compare
Pf6N2E2_1161 +0.5 2.4 FIG00961921: hypothetical protein compare
Pf6N2E2_1536 +0.5 1.7 Permeases of the major facilitator superfamily compare
Pf6N2E2_552 +0.5 1.3 Uncharacterized protein ImpF compare
Pf6N2E2_4174 +0.5 4.0 Methionine ABC transporter substrate-binding protein compare
Pf6N2E2_136 +0.5 1.2 Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE compare
Pf6N2E2_2574 +0.5 1.0 Flagellar synthesis regulator FleN compare
Pf6N2E2_4479 +0.5 2.1 FIG001590: Putative conserved exported protein precursor compare
Pf6N2E2_726 +0.5 1.9 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_2279 +0.5 1.5 FIG00954981: hypothetical protein compare
Pf6N2E2_5448 +0.5 3.1 hypothetical protein compare
Pf6N2E2_3338 +0.5 1.1 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_533 +0.5 1.6 Na(+) H(+) antiporter subunit G compare
Pf6N2E2_2109 +0.5 2.6 NAD-dependent protein deacetylase of SIR2 family compare
Pf6N2E2_1319 +0.5 1.1 FIG074102: hypothetical protein compare
Pf6N2E2_451 +0.5 0.8 transcriptional regulator MvaT, P16 subunit, putative compare
Pf6N2E2_1469 +0.5 1.7 NAD-dependent formate dehydrogenase gamma subunit compare
Pf6N2E2_1431 +0.5 1.0 Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1) compare
Pf6N2E2_3855 +0.5 2.9 Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1) compare
Pf6N2E2_3665 +0.5 0.6 serine/threonine protein kinase compare
Pf6N2E2_5597 +0.5 2.3 Electron transport complex protein RnfD compare
Pf6N2E2_1021 +0.5 2.3 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare


Specific Phenotypes

For 1 genes in this experiment

For stress Sisomicin sulfate salt in Pseudomonas fluorescens FW300-N2E2

For stress Sisomicin sulfate salt across organisms