Experiment set7IT026 for Klebsiella michiganensis M5al

LB with Cephalothin sodium salt 0.16 mg/ml

Group: stress
Media: LB + Cephalothin sodium salt (0.16 mg/ml)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 27-Apr-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 1806 A6

Specific Phenotypes

For 20 genes in this experiment

For stress Cephalothin sodium salt in Klebsiella michiganensis M5al

For stress Cephalothin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
ATP-dependent RNA helicases, bacterial	1
Bacterial Cell Division	1
Bacterial Cytoskeleton	1
Beta-lactamase	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Glycine and Serine Utilization	1
Multidrug Resistance Efflux Pumps	1
Pyruvate Alanine Serine Interconversions	1
Ribosome biogenesis bacterial	1
Tn552	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Tryptophan metabolism
• Ascorbate and aldarate metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Penicillin and cephalosporin biosynthesis
• beta-Lactam resistance
• Histidine metabolism
• Tyrosine metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
D-serine degradation	3	3	3
L-serine degradation	3	3	2
L-cysteine degradation II	3	3	2
L-tryptophan degradation II (via pyruvate)	3	3	2
pseudouridine degradation	2	2	1
peptidoglycan recycling II	10	7	4
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
L-methionine biosynthesis II	6	5	2
peptidoglycan recycling I	14	14	4
glycine betaine degradation III	7	4	2
L-mimosine degradation	8	4	2
glycine betaine degradation I	8	4	2
glutathione-mediated detoxification I	8	3	2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
purine nucleobases degradation II (anaerobic)	24	17	2