Experiment set7IT025 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Lomefloxacin hydrochloride 0.0002 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_3663 +6.1 40.9 Glycosyl transferase compare
Pf6N2E2_3661 +5.7 30.8 hypothetical protein compare
Pf6N2E2_5647 +5.7 28.8 Transcriptional regulator, TetR family compare
Pf6N2E2_2822 +5.5 35.9 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_3659 +5.5 14.2 Protein fixF compare
Pf6N2E2_3603 +4.0 14.1 FIG003573: hypothetical protein compare
Pf6N2E2_3279 +3.9 17.8 FIG138315: Putative alpha helix protein compare
Pf6N2E2_3658 +3.7 21.5 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_5046 +3.7 26.7 ATP-dependent RNA helicase PA3950 conserved
Pf6N2E2_2646 +3.6 7.6 FIG00953416: hypothetical protein compare
Pf6N2E2_2507 +3.4 13.5 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_5944 +3.0 14.0 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
Pf6N2E2_5525 +2.8 21.7 Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases compare
Pf6N2E2_3899 +2.8 20.7 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_773 +2.8 15.5 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_3791 +2.7 20.6 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_2506 +2.6 21.3 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_3664 +2.6 12.5 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2510 +2.5 10.1 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_2508 +2.5 9.8 hypothetical protein compare
Pf6N2E2_3792 +2.5 15.0 Glucans biosynthesis protein G precursor compare
Pf6N2E2_445 +2.5 11.6 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_2513 +2.4 8.5 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_1298 +2.4 5.4 Xanthine transporter,putative compare
Pf6N2E2_4381 +2.4 4.1 hypothetical protein compare
Pf6N2E2_4673 +2.4 10.2 ATP-dependent 23S rRNA helicase DbpA compare
Pf6N2E2_2681 +2.3 9.6 3-phosphoglycerate kinase compare
Pf6N2E2_3908 +2.3 16.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_4454 +2.3 11.5 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_2509 +2.2 5.0 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_4047 +2.1 13.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_70 +2.1 7.7 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_4974 +2.1 12.4 DedA protein compare
Pf6N2E2_2518 +2.0 1.9 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_4593 +2.0 10.3 Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB conserved
Pf6N2E2_2425 +1.9 8.1 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3655 +1.9 14.6 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_924 +1.9 3.3 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_3085 +1.9 6.4 FIG00954614: hypothetical protein compare
Pf6N2E2_3198 +1.8 5.2 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_926 +1.8 7.0 NADH dehydrogenase (EC 1.6.99.3) compare
Pf6N2E2_5262 +1.8 6.5 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf6N2E2_4601 +1.7 3.1 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_2519 +1.7 3.1 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_4077 +1.7 3.5 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_5175 +1.7 13.4 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_5176 +1.6 10.7 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5188 +1.6 7.6 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_3029 +1.6 7.0 Holin conserved
Pf6N2E2_3179 +1.6 1.8 FIG021952: putative membrane protein compare
Pf6N2E2_5705 +1.5 4.2 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_2124 +1.5 2.3 Mg(2+) transport ATPase protein C compare
Pf6N2E2_4612 +1.4 8.1 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_5629 +1.4 2.0 Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_3656 +1.4 7.4 Probable transcription regulator Mig-14 compare
Pf6N2E2_2597 +1.4 10.7 Flagellar regulatory protein FleQ compare
Pf6N2E2_4378 +1.3 5.1 Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2) compare
Pf6N2E2_2645 +1.3 6.1 ATP-dependent DNA helicase RecQ conserved
Pf6N2E2_5733 +1.3 5.3 NLP/P60 family protein compare
Pf6N2E2_1056 +1.3 1.5 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_3199 +1.3 6.5 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_4996 +1.3 5.2 LSU m3Psi1915 methyltransferase RlmH compare
Pf6N2E2_586 +1.2 6.6 Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-) compare
Pf6N2E2_6140 +1.2 2.2 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_2683 +1.2 4.5 Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24) compare
Pf6N2E2_3346 +1.2 6.0 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_4631 +1.2 7.7 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_5236 +1.2 4.5 hypothetical protein compare
Pf6N2E2_2526 +1.2 5.7 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_3030 +1.2 6.5 hypothetical protein compare
Pf6N2E2_3516 +1.2 2.2 Probable transmembrane protein compare
Pf6N2E2_4382 +1.2 4.7 FIG00953403: hypothetical protein compare
Pf6N2E2_3847 +1.2 4.5 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_4204 +1.1 2.7 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_4052 +1.1 2.6 FIG00955481: hypothetical protein compare
Pf6N2E2_2872 +1.1 8.1 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_4464 +1.1 3.9 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_4654 +1.1 3.9 hypothetical protein compare
Pf6N2E2_4658 +1.1 3.2 FIG00955006: hypothetical protein compare
Pf6N2E2_4635 +1.1 6.9 ATP-dependent RNA helicase RhlE compare
Pf6N2E2_2821 +1.1 3.4 hypothetical protein compare
Pf6N2E2_5794 +1.1 2.9 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_5574 +1.0 7.1 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_6100 +1.0 2.6 tRNA-Val-TAC compare
Pf6N2E2_3338 +1.0 2.0 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_5150 +1.0 5.1 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_3349 +1.0 2.3 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_1566 +1.0 2.5 FIG00960770: hypothetical protein compare
Pf6N2E2_4594 +1.0 3.9 Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15) compare
Pf6N2E2_2682 +1.0 2.1 Transcriptional regulator, LysR family compare
Pf6N2E2_2375 +1.0 2.4 Response regulator NasT compare
Pf6N2E2_2674 +1.0 1.2 FIG00953856: hypothetical protein compare
Pf6N2E2_1424 +1.0 1.2 transcriptional regulator, TetR family compare
Pf6N2E2_4993 +1.0 2.7 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) compare
Pf6N2E2_3255 +1.0 3.2 YrbA protein compare
Pf6N2E2_145 +1.0 6.7 putative exported protein compare
Pf6N2E2_2586 +1.0 2.4 Hpt domain protein compare
Pf6N2E2_2503 +1.0 1.5 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_37 +1.0 3.4 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_1147 +0.9 1.9 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_4678 +0.9 1.2 hypothetical protein compare
Pf6N2E2_5761 +0.9 2.6 Transcriptional regulator, TetR family compare
Pf6N2E2_3830 +0.9 4.1 Glutaredoxin 3 (Grx3) compare
Pf6N2E2_971 +0.9 1.8 hypothetical protein compare
Pf6N2E2_144 +0.9 7.6 Uncharacterized protein YtfM precursor compare
Pf6N2E2_55 +0.9 2.4 Probable hydrolase compare
Pf6N2E2_3770 +0.9 1.4 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_4838 +0.9 6.0 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
Pf6N2E2_2384 +0.9 2.5 CmpX compare
Pf6N2E2_282 +0.9 1.3 GNAT family acetyltransferase YjcF compare
Pf6N2E2_1339 +0.9 1.6 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_3794 +0.9 4.6 Proline iminopeptidase (EC 3.4.11.5) compare
Pf6N2E2_1759 +0.9 3.1 uncharacterized domain 1 compare
Pf6N2E2_5976 +0.9 2.1 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21) compare
Pf6N2E2_5030 +0.9 4.6 Transcriptional regulator, AsnC family compare
Pf6N2E2_5226 +0.9 1.9 FIG00955589: hypothetical protein compare
Pf6N2E2_238 +0.9 1.3 hypothetical protein compare
Pf6N2E2_2624 +0.9 5.0 Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-) compare
Pf6N2E2_4821 +0.9 4.0 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf6N2E2_4477 +0.9 3.5 Z-ring-associated protein ZapA compare
Pf6N2E2_3625 +0.8 5.6 Flagellar motor rotation protein MotA compare
Pf6N2E2_5666 +0.8 5.3 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_3285 +0.8 2.4 Rod shape-determining protein MreD compare
Pf6N2E2_4773 +0.8 1.4 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_3876 +0.8 3.0 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_4414 +0.8 1.9 FIG00953808: hypothetical protein compare
Pf6N2E2_1534 +0.8 1.5 Probable transmembrane protein compare
Pf6N2E2_2594 +0.8 4.5 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_2592 +0.8 2.6 Flagellar motor switch protein FliG compare
Pf6N2E2_5990 +0.8 1.3 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_2999 +0.8 2.6 probable tail fiber assembly protein compare
Pf6N2E2_3517 +0.8 2.8 tRNA dihydrouridine synthase B (EC 1.-.-.-) compare
Pf6N2E2_2278 +0.8 6.4 Transcription-repair coupling factor compare
Pf6N2E2_4764 +0.8 3.1 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_2264 +0.8 2.5 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf6N2E2_2065 +0.8 2.9 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_122 +0.8 1.8 Tyrosine-protein kinase Wzc (EC 2.7.10.2) compare
Pf6N2E2_6095 +0.8 1.8 tRNA-Asn-GTT compare
Pf6N2E2_2585 +0.8 4.6 Flagellar hook-length control protein FliK compare
Pf6N2E2_2281 +0.8 1.2 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_5689 +0.8 3.0 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_4761 +0.8 5.5 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_1099 +0.8 1.5 Hypothetical protein GlcG in glycolate utilization operon compare
Pf6N2E2_1959 +0.8 1.7 D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) compare
Pf6N2E2_4759 +0.8 5.9 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_1040 +0.7 1.6 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_4763 +0.7 4.7 Biotin synthesis protein BioC compare
Pf6N2E2_3237 +0.7 2.6 hypothetical protein compare
Pf6N2E2_1667 +0.7 2.2 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_1031 +0.7 2.0 Alkanesulfonates transport system permease protein compare
Pf6N2E2_5665 +0.7 4.7 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_5232 +0.7 4.4 Cell division inhibitor compare
Pf6N2E2_1699 +0.7 1.4 FIG00955832: hypothetical protein compare
Pf6N2E2_3026 +0.7 3.6 Baseplate assembly protein W compare
Pf6N2E2_1220 +0.7 2.8 hypothetical protein compare
Pf6N2E2_4809 +0.7 6.2 COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases compare
Pf6N2E2_3084 +0.7 2.2 Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51) compare
Pf6N2E2_2588 +0.7 1.6 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_3829 +0.7 3.4 Protein export cytoplasm chaperone protein (SecB, maintains protein to be exported in unfolded state) compare
Pf6N2E2_5671 +0.7 2.9 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_2788 +0.7 1.7 Transcriptional regulator, AsnC family compare
Pf6N2E2_667 +0.7 1.9 Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-) compare
Pf6N2E2_251 +0.7 2.4 transcriptional regulator compare
Pf6N2E2_1325 +0.7 1.7 FIG00954773: hypothetical protein compare
Pf6N2E2_2223 +0.7 2.0 hypothetical protein compare
Pf6N2E2_6029 +0.7 2.1 hypothetical protein compare
Pf6N2E2_4048 +0.7 4.1 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_311 +0.7 2.9 hypothetical protein compare
Pf6N2E2_2582 +0.7 2.1 Flagellar motor switch protein FliN compare
Pf6N2E2_4640 +0.7 2.0 hypothetical protein compare
Pf6N2E2_689 +0.7 2.1 FIG00956189: hypothetical protein compare
Pf6N2E2_642 +0.7 1.9 Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system compare
Pf6N2E2_1536 +0.7 2.3 Permeases of the major facilitator superfamily compare
Pf6N2E2_5691 +0.7 3.4 Flagellar basal-body P-ring formation protein FlgA compare
Pf6N2E2_3328 +0.7 1.0 transcriptional regulator compare
Pf6N2E2_5956 +0.7 3.4 FIG004453: protein YceG like compare
Pf6N2E2_953 +0.7 1.4 FIG00958851: hypothetical protein compare
Pf6N2E2_2549 +0.7 0.9 hypothetical protein compare
Pf6N2E2_4540 +0.7 2.6 hypothetical protein compare
Pf6N2E2_5190 +0.7 4.7 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_1806 +0.7 1.5 hypothetical protein compare
Pf6N2E2_2590 +0.7 5.3 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_603 +0.7 1.7 FIG00953934: hypothetical protein compare
Pf6N2E2_5560 +0.7 2.4 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf6N2E2_2593 +0.7 3.4 Flagellar M-ring protein FliF compare
Pf6N2E2_2903 +0.7 1.9 Heme oxygenase HemO, associated with heme uptake compare
Pf6N2E2_3624 +0.7 3.5 Flagellar motor rotation protein MotB compare
Pf6N2E2_5927 +0.7 2.7 ABC-type multidrug transport system, ATPase component compare
Pf6N2E2_3672 +0.7 4.1 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare
Pf6N2E2_1975 +0.7 2.6 Integrase regulator R compare
Pf6N2E2_5819 +0.6 3.2 Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p compare
Pf6N2E2_1617 +0.6 1.0 COG2199: FOG: GGDEF domain compare
Pf6N2E2_5697 +0.6 5.0 Flagellar hook protein FlgE compare
Pf6N2E2_3535 +0.6 3.2 Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) compare
Pf6N2E2_3600 +0.6 4.9 Fe-S OXIDOREDUCTASE (1.8.-.-) compare
Pf6N2E2_3546 +0.6 2.8 ABC transporter (iron.B12.siderophore.hemin) , permease component compare
Pf6N2E2_572 +0.6 1.6 hypothetical protein compare
Pf6N2E2_1602 +0.6 2.2 hypothetical protein compare
Pf6N2E2_5667 +0.6 3.9 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_2600 +0.6 4.9 Flagellar hook-associated protein FliD compare


Specific Phenotypes

For 29 genes in this experiment

For stress Lomefloxacin hydrochloride in Pseudomonas fluorescens FW300-N2E2

For stress Lomefloxacin hydrochloride across organisms