Experiment set7IT025 for Pseudomonas fluorescens FW300-N2E2

LB with Lomefloxacin hydrochloride 0.0002 mM

Group: stress
Media: LB + Lomefloxacin hydrochloride (2e-04 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Mark on 2/1/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 29 genes in this experiment

For stress Lomefloxacin hydrochloride in Pseudomonas fluorescens FW300-N2E2

For stress Lomefloxacin hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	3
DNA-replication	2
ABC transporter dipeptide (TC 3.A.1.5.2)	1
ATP-dependent RNA helicases, bacterial	1
Arginine and Ornithine Degradation	1
Bacterial Chemotaxis	1
Carboxysome	1
DNA repair, bacterial DinG and relatives	1
DNA repair, bacterial RecFOR pathway	1
Heat shock dnaK gene cluster extended	1
Heme and Siroheme Biosynthesis	1
Lipid A modifications	1
Methionine Biosynthesis	1
Methionine Degradation	1
Methionine Salvage	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Peptidoglycan Biosynthesis	1
Putative TldE-TldD proteolytic complex	1
Queuosine-Archaeosine Biosynthesis	1
Respiratory dehydrogenases 1	1
Staphylococcal pathogenicity islands SaPI	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Peptidoglycan biosynthesis
• Oxidative phosphorylation
• Urea cycle and metabolism of amino groups
• Methionine metabolism
• Lysine degradation
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Diterpenoid biosynthesis
• Alkaloid biosynthesis I
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
NADH to cytochrome bo oxidase electron transfer I	2	2	1
phenylethylamine degradation I	2	2	1
NADH to cytochrome bd oxidase electron transfer I	2	1	1
aerobic respiration III (alternative oxidase pathway)	3	3	1
L-tryptophan degradation VI (via tryptamine)	3	1	1
5'-deoxyadenosine degradation I	3	1	1
noradrenaline and adrenaline degradation	13	8	4
aerobic respiration I (cytochrome c)	4	3	1
putrescine degradation III	4	3	1
aromatic biogenic amine degradation (bacteria)	8	4	2
L-tryptophan degradation X (mammalian, via tryptamine)	4	2	1
salidroside biosynthesis	4	2	1
melatonin degradation II	4	1	1
5'-deoxyadenosine degradation II	4	1	1
dopamine degradation	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III	5	2	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II	5	2	1
phenylethanol biosynthesis	5	2	1
NAD(P)/NADPH interconversion	6	3	1
Fe(II) oxidation	6	3	1
serotonin degradation	7	4	1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I	7	2	1
peptidoglycan biosynthesis II (staphylococci)	17	12	2
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	2
peptidoglycan biosynthesis V (β-lactam resistance)	17	11	2
L-phenylalanine degradation IV (mammalian, via side chain)	9	5	1
superpathway of phenylethylamine degradation	11	6	1
L-methionine salvage cycle III	11	5	1
L-methionine salvage cycle II (plants)	11	3	1
superpathway of melatonin degradation	11	1	1
peptidoglycan biosynthesis I (meso-diaminopimelate containing)	12	12	1
L-methionine salvage cycle I (bacteria and plants)	12	4	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	1
peptidoglycan biosynthesis III (mycobacteria)	15	11	1