Experiment set7IT024 for Rhodanobacter denitrificans FW104-10B01

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Anaerobic, acetate donor; nitrate and iron electron acceptors

Group: anaerobic
Media: Hans_Basal_Media_bicarb_nitrate + Potassium acetate (5 mM) + Iron (II) chloride tetrahydrate (10 mM mM), pH=7
Culturing: rhodanobacter_10B01_ML12, tube, Anaerobic, at 30 (C)
By: Hans Carlson on 09/22/2021
Media components: 2.5 g/L Sodium bicarbonate, 0.1 g/L Potassium Chloride, 0.01 g/L Sodium Chloride, 0.01 g/L Calcium chloride dihydrate, 0.1 g/L Magnesium chloride hexahydrate, 0.1 g/L Sodium sulfate, 0.25 g/L Ammonium chloride, 0.1 g/L Disodium phosphate, 10 mM Sodium nitrate, DL vitamins (0.0002 mg/L biotin, 0.0002 mg/L Folic Acid, 0.001 mg/L Pyridoxine HCl, 0.0005 mg/L Riboflavin, 0.0005 mg/L Thiamine HCl, 0.0005 mg/L Nicotinic Acid, 0.0005 mg/L calcium pantothenate, 1e-05 mg/L Cyanocobalamin, 0.0005 mg/L 4-Aminobenzoic acid, 0.0005 mg/L Lipoic acid), Sulfur-free DL minerals (0.0003 g/L Magnesium chloride hexahydrate, 0.00015 g/L Nitrilotriacetic acid disodium salt, 0.0001 g/L Sodium Chloride, 5e-05 g/L Manganese (II) chloride tetrahydrate, 1e-05 g/L Cobalt chloride hexahydrate, 1.3e-05 g/L Zinc chloride, 1e-05 g/L Calcium chloride dihydrate, 1e-05 g/L Iron (II) chloride tetrahydrate, 2.5e-06 g/L Nickel (II) chloride hexahydrate, 2e-06 g/L Aluminum chloride hydrate, 1e-06 g/L copper (II) chloride dihydrate, 1e-06 g/L Boric Acid, 1e-06 g/L Sodium Molybdate Dihydrate, 3e-05 g/L Sodium selenite pentahydrate, 2.5e-05 g/L Sodium tungstate dihydrate)

Specific Phenotypes

For 52 genes in this experiment

For anaerobic Potassium acetate in Rhodanobacter denitrificans FW104-10B01

For anaerobic Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 6
Nitrate and nitrite ammonification 3
Oxidative stress 3
Transport of Molybdenum 3
Formate hydrogenase 2
Type IV pilus 2
Autoinducer 2 (AI-2) transport and processing (lsrACDBFGE operon) 1
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
DNA-binding regulatory proteins, strays 1
Experimental tye 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Heme and Siroheme Biosynthesis 1
Hydrogenases 1
Ketoisovalerate oxidoreductase 1
Methionine Biosynthesis 1
Methionine Degradation 1
Orphan regulatory proteins 1
Protein degradation 1
Pyruvate Alanine Serine Interconversions 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Respiratory dehydrogenases 1 1
Serine-glyoxylate cycle 1
Soluble cytochromes and functionally related electron carriers 1
Terminal cytochrome C oxidases 1
Thiamin biosynthesis 1
Thioredoxin-disulfide reductase 1
Ubiquinone Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
nitrate reduction IX (dissimilatory) 2 2 1
L-alanine degradation I 2 2 1
3-dehydroquinate biosynthesis I 2 2 1
nitrate reduction III (dissimilatory) 2 1 1
NADH to nitrate electron transfer 2 1 1
arsenite to oxygen electron transfer 2 1 1
nitrate reduction VIIIb (dissimilatory) 2 1 1
nitrate reduction VIII (dissimilatory) 2 1 1
superpathway of acetate utilization and formation 3 3 1
ethanol degradation IV 3 3 1
ethanol degradation II 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
heme b biosynthesis II (oxygen-independent) 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
S-adenosyl-L-methionine salvage I 4 3 1
chitin deacetylation 4 2 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
diphenyl ethers degradation 4 2 1
ubiquinol-8 biosynthesis (late decarboxylation) 9 8 2
nitrate reduction I (denitrification) 5 4 1
nitrate reduction VII (denitrification) 5 4 1
2-methylcitrate cycle I 5 4 1
autoinducer AI-2 biosynthesis I 5 3 1
biphenyl degradation 5 2 1
glyoxylate cycle 6 6 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of heme b biosynthesis from uroporphyrinogen-III 6 4 1
autoinducer AI-2 biosynthesis II (Vibrio) 6 4 1
thiazole component of thiamine diphosphate biosynthesis I 6 3 1
2-methylcitrate cycle II 6 3 1
molybdopterin biosynthesis 6 2 1
Fe(II) oxidation 6 2 1
superpathway of bitter acids biosynthesis 18 3 3
colupulone and cohumulone biosynthesis 6 1 1
β-alanine biosynthesis II 6 1 1
lupulone and humulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
chorismate biosynthesis I 7 7 1
thiazole component of thiamine diphosphate biosynthesis II 7 4 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 4 1
ubiquinol-8 biosynthesis (early decarboxylation) 8 6 1
ubiquinol-6 biosynthesis (late decarboxylation) 8 3 1
ubiquinol-9 biosynthesis (early decarboxylation) 8 2 1
ubiquinol-7 biosynthesis (late decarboxylation) 8 2 1
ubiquinol-7 biosynthesis (early decarboxylation) 8 2 1
ubiquinol-10 biosynthesis (early decarboxylation) 8 2 1
ubiquinol-9 biosynthesis (late decarboxylation) 8 2 1
reductive glycine pathway of autotrophic CO2 fixation 9 5 1
ubiquinol-6 biosynthesis from 4-aminobenzoate (yeast) 9 3 1
ubiquinol-10 biosynthesis (late decarboxylation) 9 2 1
3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) 9 2 1
cis-geranyl-CoA degradation 9 2 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-phenylalanine biosynthesis 10 10 1
glycolysis V (Pyrococcus) 10 7 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 1
superpathway of thiamine diphosphate biosynthesis I 10 5 1
glycolysis II (from fructose 6-phosphate) 11 11 1
superpathway of thiamine diphosphate biosynthesis II 11 6 1
superpathway of ubiquinol-6 biosynthesis (late decarboxylation) 11 4 1
superpathway of glyoxylate bypass and TCA 12 10 1
superpathway of ubiquinol-8 biosynthesis (early decarboxylation) 12 10 1
superpathway of L-tryptophan biosynthesis 13 13 1
glycolysis I (from glucose 6-phosphate) 13 12 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 23 2
gluconeogenesis I 13 11 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 16 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of hexitol degradation (bacteria) 18 14 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 8 1
superpathway of anaerobic sucrose degradation 19 16 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 16 1
superpathway of bacteriochlorophyll a biosynthesis 26 5 1
superpathway of chorismate metabolism 59 40 2
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 25 1