Experiment set7IT024 for Sinorhizobium meliloti 1021

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Plant=None; PlantTreatment=None; Sample=no_plant_control; GrowthSubstrate=agar_0.75vol%; Collection=direct (sample tracking unclear; do not publish)

Group: in planta
Media: Modified_MS_plus_RCH2_0.2x_each_no_Nitrate + Plant=None; PlantTreatment=None; Sample=no_plant_control; GrowthSubstrate=agar_0.75vol%; Collection=direct + D-Glucose (10 mM)
Culturing: Smeli_ML6a, pot, Aerobic, at 26 (C), (Solid)
By: Trenton on 14-Mar-19
Media components: 1.55 mg/L Boric Acid, 83.05 mg/L Calcium chloride, 0.00625 mg/L Cobalt chloride hexahydrate, 0.00625 mg/L Copper (II) sulfate pentahydrate, 9.315 mg/L EDTA (disodium salt), 6.95 mg/L Iron (II) sulfate heptahydrate, 45.175 mg/L Magnesium sulfate, 4.225 mg/L Manganese sulfate, 0.2075 mg/L Potassium iodide, 42.5 mg/L Potassium phosphate monobasic, 0.0625 mg/L Sodium Molybdate Dihydrate, 2.15 mg/L Zinc sulfate heptahydrate, 5 g/L Ammonium chloride, 0.02 g/L Potassium Chloride, 0.12 g/L Sodium phosphate monobasic monohydrate, 6 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.006 g/L Magnesium Sulfate Heptahydrate, 0.003 g/L Nitrilotriacetic acid, 0.002 g/L Sodium Chloride, 0.001 g/L Manganese (II) sulfate monohydrate, 0.0002 g/L Cobalt chloride hexahydrate, 0.0002 g/L Zinc sulfate heptahydrate, 0.0002 g/L Calcium chloride dihydrate, 0.0002 g/L Iron (II) sulfate heptahydrate, 5e-05 g/L Nickel (II) chloride hexahydrate, 4e-05 g/L Aluminum potassium sulfate dodecahydrate, 2e-05 g/L Copper (II) sulfate pentahydrate, 2e-05 g/L Boric Acid, 2e-05 g/L Sodium Molybdate Dihydrate, 0.0006 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.02 mg/L Pyridoxine HCl, 0.01 mg/L 4-Aminobenzoic acid, 0.01 mg/L Lipoic acid, 0.01 mg/L Nicotinic Acid, 0.01 mg/L Riboflavin, 0.01 mg/L Thiamine HCl, 0.01 mg/L calcium pantothenate, 0.004 mg/L biotin, 0.004 mg/L Folic Acid, 0.0002 mg/L Cyanocobalamin)

Specific Phenotypes

For 11 genes in this experiment

For in planta Plant=None; PlantTreatment=None; Sample=no_plant_control; GrowthSubstrate=agar_0.75vol%; Collection=direct in Sinorhizobium meliloti 1021

For in planta Plant=None; PlantTreatment=None; Sample=no_plant_control; GrowthSubstrate=agar_0.75vol%; Collection=direct across organisms

SEED Subsystems

Subsystem #Specific
DNA-replication 2
Two cell division clusters relating to chromosome partitioning 2
DNA repair, bacterial RecFOR pathway 1
Flagellum 1
Transcription factors bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes: